DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertools

A Jha, SC Bohaczuk, Y Mao, J Ranchalis… - Genome …, 2024 - genome.cshlp.org
Long-read DNA sequencing has recently emerged as a powerful tool for studying both
genetic and epigenetic architectures at single-molecule and single-nucleotide resolution …

Examining chromatin heterogeneity through PacBio long-read sequencing of M.EcoGII methylated genomes: an m6A detection efficiency and calling bias correcting …

AF Dennis, Z Xu, DJ Clark - Nucleic Acids Research, 2024 - academic.oup.com
Recent studies have combined DNA methyltransferase footprinting of genomic DNA in
nuclei with long-read sequencing, resulting in detailed chromatin maps for multi-kilobase …

Maternal SMARCA5 is required for major ZGA in mouse embryos

ON Kubinyecz, D Drage, F Santos, C Krueger… - bioRxiv, 2023 - biorxiv.org
Zygotic genome activation (ZGA) in mice takes place in two waves, a minor wave in the one-
cell embryo, and a major wave at the two-cell stage, both accompanied by global …

Sensitive multimodal profiling of native DNA by transposase-mediated single-molecule sequencing

AS Nanda, K Wu, S Kasinathan, MS Ostrowski… - bioRxiv, 2022 - biorxiv.org
We present SMRT-Tag: a multiplexable, PCR-free approach for constructing low-input,
single-molecule Pacific Biosciences (PacBio) sequencing libraries through Tn5 …

The single-molecule accessibility landscape of newly replicated mammalian chromatin

MS Ostrowski, MG Yang, CP McNally, NJ Abdulhay… - Cell, 2025 - cell.com
We present replication-aware single-molecule accessibility map** (RASAM), a method to
nondestructively measure replication status and protein-DNA interactions on chromatin …

Remodeling of higher order chromatin structures

P Vizjak - 2023 - edoc.ub.uni-muenchen.de
In fast allen Eukaryoten ist die DNA in einem dynamischen Polymer namens Chromatin
organisiert. Das Nukleosom, der elementare Baustein des Chromatins, besteht aus einem …