Good vibrations in enzyme-catalysed reactions
Fast motions (femtosecond to picosecond) and their potential involvement during enzyme-
catalysed reactions have ignited considerable interest in recent years. Their influence on …
catalysed reactions have ignited considerable interest in recent years. Their influence on …
Biocatalytic reductions and chemical versatility of the old yellow enzyme family of flavoprotein oxidoreductases
The old yellow enzyme (OYE) family is a large group of flavin‐dependent redox biocatalysts
with major applications in the industrial reduction of activated alkenes. These enzymes have …
with major applications in the industrial reduction of activated alkenes. These enzymes have …
A 21st century revisionist's view at a turning point in enzymology
ZD Nagel, JP Klinman - Nature chemical biology, 2009 - nature.com
Despite the fact that the number of publications associated with the keyword'enzyme'
increases every year, the precise origin of enzyme catalysis has remained unresolved …
increases every year, the precise origin of enzyme catalysis has remained unresolved …
Old yellow enzyme-catalysed asymmetric hydrogenation: linking family roots with improved catalysis
A Scholtissek, D Tischler, AH Westphal… - Catalysts, 2017 - mdpi.com
Asymmetric hydrogenation of activated alkenes catalysed by ene-reductases from the old
yellow enzyme family (OYEs) leading to chiral products is of potential interest for industrial …
yellow enzyme family (OYEs) leading to chiral products is of potential interest for industrial …
Decoupling of catalysis and transition state analog binding from mutations throughout a phosphatase revealed by high-throughput enzymology
Using high-throughput microfluidic enzyme kinetics (HT-MEK), we measured over 9,000
inhibition curves detailing impacts of 1,004 single-site mutations throughout the alkaline …
inhibition curves detailing impacts of 1,004 single-site mutations throughout the alkaline …
Biocatalysis with thermostable enzymes: structure and properties of a thermophilic 'ene'‐reductase related to old yellow enzyme
We report the crystal structure of a thermophilic “ene” reductase (TOYE) isolated from
Thermoanaerobacter pseudethanolicus E39. The crystal structure reveals a tetrameric …
Thermoanaerobacter pseudethanolicus E39. The crystal structure reveals a tetrameric …
Hydrogen donor–acceptor fluctuations from kinetic isotope effects: A phenomenological model
Kinetic isotope effects (KIEs) and their temperature dependence can probe the structural
and dynamic nature of enzyme-catalyzed proton or hydride transfers. The molecular …
and dynamic nature of enzyme-catalyzed proton or hydride transfers. The molecular …
Direct Analysis of Donor− Acceptor Distance and Relationship to Isotope Effects and the Force Constant for Barrier Compression in Enzymatic H-Tunneling Reactions
The role of dynamical effects in enzyme catalysis is both complex and widely debated.
Understanding how dynamics can influence the barrier to an enzyme catalyzed reaction …
Understanding how dynamics can influence the barrier to an enzyme catalyzed reaction …
Evidence to support the hypothesis that promoting vibrations enhance the rate of an enzyme catalyzed H-tunneling reaction
In recent years there has been a shift away from transition state theory models for H-transfer
reactions. Models that incorporate tunneling as the mechanism of H-transfer are now …
reactions. Models that incorporate tunneling as the mechanism of H-transfer are now …
Deep tunneling dominates the biologically important hydride transfer reaction from NADH to FMN in morphinone reductase
The temperature dependence of the primary kinetic isotope effect (KIE), combined
temperature− pressure studies of the primary KIE, and studies of the α-secondary KIE …
temperature− pressure studies of the primary KIE, and studies of the α-secondary KIE …