Controlling reaction specificity in pyridoxal phosphate enzymes

MD Toney - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2011 - Elsevier
Pyridoxal 5′-phosphate enzymes are ubiquitous in the nitrogen metabolism of all
organisms. They catalyze a wide variety of reactions including racemization, transamination …

Aspartate aminotransferase: an old dog teaches new tricks

MD Toney - Archives of biochemistry and biophysics, 2014 - Elsevier
Aspartate aminotransferase (AAT) is a prototypical pyridoxal 5′-phosphate (PLP)
dependent enzyme that catalyzes the reversible interconversion of l-aspartate and α …

Aromatic L-amino acid decarboxylases: mechanistic features and microbial applications

SW Han, JS Shin - Applied Microbiology and Biotechnology, 2022 - Springer
Aromatic L-amino acid decarboxylases (AADCs) catalyze the conversion of aromatic L-
amino acids into aromatic monoamines that play diverse physiological and biosynthetic …

NMR crystallography of a carbanionic intermediate in tryptophan synthase: chemical structure, tautomerization, and reaction specificity

BG Caulkins, RP Young, RA Kudla… - Journal of the …, 2016 - ACS Publications
Carbanionic intermediates play a central role in the catalytic transformations of amino acids
performed by pyridoxal-5′-phosphate (PLP)-dependent enzymes. Here, we make use of …

Direct visualization of critical hydrogen atoms in a pyridoxal 5′-phosphate enzyme

S Dajnowicz, RC Johnston, JM Parks… - Nature …, 2017 - nature.com
Enzymes dependent on pyridoxal 5′-phosphate (PLP, the active form of vitamin B6)
perform a myriad of diverse chemical transformations. They promote various reactions by …

Role of the pyridine nitrogen in pyridoxal 5′-phosphate catalysis: activity of three classes of PLP enzymes reconstituted with deazapyridoxal 5′-phosphate

WR Griswold, MD Toney - Journal of the American Chemical …, 2011 - ACS Publications
Pyridoxal 5′-phosphate (PLP; vitamin B6)-catalyzed reactions have been well studied, both
on enzymes and in solution, due to the variety of important reactions this cofactor catalyzes …

Direct evidence that an extended hydrogen-bonding network influences activation of pyridoxal 5′-phosphate in aspartate aminotransferase

S Dajnowicz, JM Parks, X Hu, K Gesler… - Journal of Biological …, 2017 - jbc.org
Pyridoxal 5′-phosphate (PLP) is a fundamental, multifunctional enzyme cofactor used to
catalyze a wide variety of chemical reactions involved in amino acid metabolism. PLP …

Experimental and molecular modeling investigation of (E)-N-{2-[(2-hydroxybenzylidene)amino]phenyl}benzenesulfonamide

N Özdemir, S Dayan, O Dayan, M Dinçer… - Molecular …, 2013 - Taylor & Francis
The Schiff base compound (E)-N-{2-[(2-hydroxybenzylidene) amino] phenyl}
benzenesulfonamide has been synthesized and characterized by IR, NMR and Uv-vis …

Site-selective deuteration of α-amino esters with 2-hydroxynicotinaldehyde as a catalyst

J Zheng, J Tang, S **, H Hu, ZJ Jiang, J Chen… - ACS …, 2024 - ACS Publications
An efficient method has been developed for the synthesis of α-deuterated α-amino esters via
hydrogen isotope exchange of α-amino esters in D2O with 2-hydroxynicotinaldehyde as a …