Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection

AP Demchenko - Methods and applications in fluorescence, 2020 - iopscience.iop.org
Photochemical stability is one of the most important parameters that determine the
usefulness of organic dyes in different applications. This Review addresses key factors that …

[HTML][HTML] Image analysis and machine learning for detecting malaria

M Poostchi, K Silamut, RJ Maude, S Jaeger… - Translational …, 2018 - Elsevier
Malaria remains a major burden on global health, with roughly 200 million cases worldwide
and more than 400,000 deaths per year. Besides biomedical research and political efforts …

Accuracy and precision in quantitative fluorescence microscopy

JC Waters - 2009 - rupress.org
The light microscope has long been used to document the localization of fluorescent
molecules in cell biology research. With advances in digital cameras and the discovery and …

Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution

EH Rego, L Shao, JJ Macklin, L Winoto… - Proceedings of the …, 2012 - pnas.org
Using ultralow light intensities that are well suited for investigating biological samples, we
demonstrate whole-cell superresolution imaging by nonlinear structured-illumination …

Revealing the dependency of dye adsorption and photocatalytic activity of ZnO nanoparticles on their morphology and defect states

Y Hendrix, E Rauwel, K Nagpal, R Haddad… - Nanomaterials, 2023 - mdpi.com
ZnO is an effective photocatalyst applied to the degradation of organic dyes in aqueous
media. In this study, the UV-light and sunlight-driven photocatalytic activities of ZnO …

Lipid droplets as endogenous intracellular microlenses

X Chen, T Wu, Z Gong, J Guo, X Liu, Y Zhang… - Light: Science & …, 2021 - nature.com
Using a single biological element as a photonic component with well-defined features has
become a new intriguing paradigm in biophotonics. Here we show that endogenous lipid …

Reactive species involved in the regioselective photooxidation of heptamethine cyanines

RR Nani, JA Kelley, J Ivanic, MJ Schnermann - Chemical science, 2015 - pubs.rsc.org
Heptamethine cyanines are important near-IR fluorophores used in many fluorescence
applications. Despite this utility, these molecules are susceptible to light-promoted reactions …

Neural network-based processing and reconstruction of compromised biophotonic image data

MJ Fanous, P Casteleiro Costa, Ç Işıl… - Light: Science & …, 2024 - nature.com
In recent years, the integration of deep learning techniques with biophotonic setups has
opened new horizons in bioimaging. A compelling trend in this field involves deliberately …

Designing a rigorous microscopy experiment: Validating methods and avoiding bias

APT Jost, JC Waters - Journal of Cell Biology, 2019 - rupress.org
Images generated by a microscope are never a perfect representation of the biological
specimen. Microscopes and specimen preparation methods are prone to error and can …

Standards and methodologies for characterizing radiobiological impact of high-Z nanoparticles

A Subiel, R Ashmore, G Schettino - Theranostics, 2016 - pmc.ncbi.nlm.nih.gov
Research on the application of high-Z nanoparticles (NPs) in cancer treatment and
diagnosis has recently been the subject of growing interest, with much promise being shown …