In a biological system, the spatiotemporal arrangement of enzymes in a dense cellular
milieu, subcellular compartments, membrane-associated enzyme complexes on cell …

Access to efficient enzymatic channeling is desired for improving all manner of designer
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …

Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …

Interactions of plasmonic nanocolloids such as gold nanoparticles and nanorods with
proximal dye emitters result in efficient quenching of the dye photoluminescence (PL). This …

The natural micro‐and nanoscale organization of biomacromolecules is a remarkable
principle within living cells, allowing for the control of cellular functions by …

Measuring the activity of low-abundance enzymes, down to a few molecules in one living
cell, is important but challenging to elucidate their biological function. Here, an …

Synthetic biology is touted as the next industrial revolution as it promises access to greener
biocatalytic syntheses to replace many industrial organic chemistries. Here, it is shown to …

Develo** reliable methods of constructing cell-free multienzyme biocatalytic systems is a
milestone goal of synthetic biology. It would enable overcoming the limitations of current cell …

Colocalization of cascade enzymes is broadly discussed as a phenomenon that can boost
the cascade reaction throughput, although a direct experimental verification is often …