Gene essentiality is a founding concept of genetics with important implications in both
fundamental and applied research. Multiple screens have been performed over the years in …

Functional genomics is the use of systematic gene perturbation approaches to determine the
contributions of genes under conditions of interest. Although functional genomic strategies …

Retrons are prokaryotic genetic retroelements encoding a reverse transcriptase that
produces multi-copy single-stranded DNA (msDNA). Despite decades of research on the …

A systems-level understanding of Gram-positive bacteria is important from both an
environmental and health perspective and is most easily obtained when high-quality …

Salmonella utilizes a type 3 secretion system to translocate virulence proteins (effectors) into
host cells during infection. The effectors modulate host cell machinery to drive uptake of the …

Bacteria can withstand killing by bactericidal antibiotics through phenotypic changes
mediated by their preexisting genetic repertoire. These changes can be exhibited transiently …

Bacteria commonly form dense biofilms encased in extracellular polymeric substances
(EPS). Biofilms are often extremely tolerant to antimicrobials but their reliance on shared …

Summary Low-molecular-weight (LMW) thiols are small-molecule antioxidants required for
the maintenance of intracellular redox homeostasis. However, many host-associated …

Internalization of pathogenic bacteria by macrophages results in formation of antibiotic-
tolerant persisters. These cells are maintained in a non-growing state for extended periods …

Key to the success of intracellular pathogens is the ability to sense and respond to a
changing host cell environment. Macrophages exposed to microbial products undergo …