The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Protein-fragment complementation assays (PCAs) are widely used for investigating protein
interactions. However, the fragments used are structurally compromised and have not been …

The N‐end rule relates the regulation of the in vivo half‐life of a protein to the identity of its N‐
terminal residue. Degradation signals (degrons) that are targeted by the N‐end rule pathway …

Since the discovery of lysosomes more than 70 years ago, much has been learned about
the functions of these organelles. Lysosomes were regarded as exclusively degradative …

Protein interactions are a fundamental mechanism for the generation of biological regulatory
specificity. The study of protein interactions in living cells is of particular significance …

Eukaryotic cells are organized into membrane-bound organelles. These organelles
communicate with one another through vesicular trafficking pathways and membrane …

Identifying key players and their interactions is fundamental for understanding biochemical
mechanisms at the molecular level. The ever-increasing number of alternative ways to …

Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of
protein interactions in living cells. The BiFC assay is based on the discoveries that two non …

The ability to make specific perturbations to biological molecules in a cell or organism is a
central experimental strategy in modern research biology. We have developed a general …