[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022 - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Environmental RNA as a tool for marine community biodiversity assessments

MS Giroux, JR Reichman, T Langknecht… - Scientific Reports, 2022 - nature.com
Microscopic organisms are often overlooked in traditional diversity assessments due to the
difficulty of identifying them based on morphology. Metabarcoding is a method for rapidly …

Analysis of small microplastics in coastal surface water samples of the subtropical island of Okinawa, Japan

C Ripken, DG Kotsifaki, SN Chormaic - Science of the total Environment, 2021 - Elsevier
Marine plastic debris is widely recognized as a global environmental issue. Small
microplastic particles, with an upper size limit of 20 μm, have been identified as having the …

Environmental DNA metabarcoding reveals and unpacks a biodiversity conservation paradox in Mediterranean marine reserves

E Boulanger, N Loiseau, A Valentini… - … of the Royal …, 2021 - royalsocietypublishing.org
Although we are currently experiencing worldwide biodiversity loss, local species richness
does not always decline under anthropogenic pressure. This conservation paradox may …

Revisiting the effect of PCR replication and sequencing depth on biodiversity metrics in environmental DNA metabarcoding

S Shirazi, RS Meyer, B Shapiro - Ecology and Evolution, 2021 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding is an increasingly popular tool for measuring
and cataloguing biodiversity. Because the environments and substrates in which DNA is …

Environmental DNA as an emerging tool in botanical research

MD Johnson, JR Freeland, L Parducci… - American journal of …, 2023 - Wiley Online Library
Over the past quarter century, environmental DNA (eDNA) has been ascendant as a tool to
detect, measure, and monitor biodiversity (species and communities), as a means of …

eDNA metabarcoding illuminates species diversity and composition of three phyla (chordata, mollusca and echinodermata) across Indonesian coral reefs

H Madduppa, NKD Cahyani, AW Anggoro… - Biodiversity and …, 2021 - Springer
Environmental DNA (eDNA) metabarcoding is an evolving tool that can provide broader
assessments of marine biodiversity than conventional visual census methods. The outputs of …

[HTML][HTML] Marine health of the Arabian Gulf: Drivers of pollution and assessment approaches focusing on desalination activities

H Hosseini, I Saadaoui, N Moheimani, M Al Saidi… - Marine Pollution …, 2021 - Elsevier
Abstract The Arabian Gulf is one of the most adversely affected marine environments
worldwide, which results from combined pollution drivers including climate change, oil and …

Systematic review of marine environmental DNA metabarcoding studies: toward best practices for data usability and accessibility

MM Shea, J Kuppermann, MP Rogers, DS Smith… - PeerJ, 2023 - peerj.com
The emerging field of environmental DNA (eDNA) research lacks universal guidelines for
ensuring data produced are FAIR–findable, accessible, interoperable, and reusable–despite …