Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Public archiving in structural biology is well established with the Protein Data Bank (PDB;
wwPDB. org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; …

Cryogenic-electron tomography enables the visualization of cellular environments in
extreme detail, however, tools to analyze the full amount of information contained within …

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological
processes in their native, cellular environment at atomic resolution. However, few cells are …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Intraflagellar transport (IFT) is the highly conserved process by which proteins are
transported along ciliary microtubules by a train-like polymeric assembly of IFT-A and IFT-B …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …