CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Forward genetic screens are powerful tools for the discovery and functional annotation of
genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short …

Cancer is a multistep disease driven by the activation of specific oncogenic pathways
concomitantly with the loss of function of tumor suppressor genes that act as sentinels to …

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the
Drosophila genome and report that Cas9-mediated genomic modifications are efficiently …

Fruit flies of the genus Drosophila have been an attractive and effective genetic model
organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them …

Image-based screening is used to measure a variety of phenotypes in cells and whole
organisms. Combined with perturbations such as RNA interference, small molecules, and …

In Darwin's and Mendel's times, researchers investigated a wealth of organisms, chosen to
solve particular problems for which they seemed especially well suited. Later, a focus on a …

With the increase in the ageing population, neurodegenerative disease is devastating to
families and poses a huge burden on society. The brain and spinal cord are extraordinarily …

Genome-editing tools, and in particular those based on CRISPR–Cas (clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein) systems, are …

Over the past decade there has been a greater understanding of genomic complexity in
eukaryotes ushered in by the immense technological advances in high-throughput …