Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

Leucine-rich repeat kinase 2 (LRRK2) is one of the most promising targets for Parkinson's
disease. LRRK2-targeting strategies have primarily focused on type 1 kinase inhibitors …

Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …

Almost 2 decades after linking LRRK2 to Parkinson's disease, a vibrant research field has
developed around the study of this gene and its protein product. Recent studies have begun …

Ribosomes translate genetic information into primary structure. During translation, various
cofactors transiently bind to the ribosome that undergoes prominent conformational and …

Intracellular phase separation is emerging as a universal principle for organizing
biochemical reactions in time and space. It remains incompletely resolved how biological …

Activating mutations in leucine-rich repeat kinase 2 (LRRK2) represent the most common
cause of monogenic Parkinson's disease. LRRK2 is a large multidomain protein kinase that …

Protein misfolding and aggregation, a key contributor to the progression of numerous
neurodegenerative diseases, results in functional deficiencies and the creation of harmful …