Structure-and mechanism-guided design of single fluorescent protein-based biosensors
Intensiometric genetically encoded biosensors, based on allosteric modulation of the
fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of …
fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of …
Harmonizing the growing fluorogenic RNA aptamer toolbox for RNA detection and imaging
X Lu, KYS Kong, PJ Unrau - Chemical Society Reviews, 2023 - pubs.rsc.org
The field of fluorogenic RNA aptamers is a burgeoning research area that aims to address
the lack of naturally fluorescent RNA molecules for RNA detection and imaging. These small …
the lack of naturally fluorescent RNA molecules for RNA detection and imaging. These small …
PARylated PDHE1α generates acetyl-CoA for local chromatin acetylation and DNA damage repair
Chromatin relaxation is a prerequisite for the DNA repair machinery to access double-strand
breaks (DSBs). Local histones around the DSBs then undergo prompt changes in …
breaks (DSBs). Local histones around the DSBs then undergo prompt changes in …
Metabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction
CM François, T Pihl, M Dunoyer de Segonzac… - Nature …, 2023 - nature.com
The molecular mechanisms connecting cellular metabolism with differentiation remain
poorly understood. Here, we find that metabolic signals contribute to stem cell differentiation …
poorly understood. Here, we find that metabolic signals contribute to stem cell differentiation …
Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress
The most abundant cellular divalent cations, Mg 2+(mM) and Ca 2+(nM-μM),
antagonistically regulate divergent metabolic pathways with several orders of magnitude …
antagonistically regulate divergent metabolic pathways with several orders of magnitude …
Maximizing the performance of protein-based fluorescent biosensors
Fluorescent protein (FP)-based biosensors are genetically encoded tools that enable the
imaging of biological processes in the context of cells, tissues, or live animals. Though …
imaging of biological processes in the context of cells, tissues, or live animals. Though …
Genetically encoded tools for measuring and manipulating metabolism
M Choe, DV Titov - Nature chemical biology, 2022 - nature.com
Over the past few years, we have seen an explosion of novel genetically encoded tools for
measuring and manipulating metabolism in live cells and animals. Here, we will review the …
measuring and manipulating metabolism in live cells and animals. Here, we will review the …
From isles of Königsberg to islets of Langerhans: examining the function of the endocrine pancreas through network science
Islets of Langerhans are multicellular microorgans located in the pancreas that play a central
role in whole-body energy homeostasis. Through secretion of insulin and other hormones …
role in whole-body energy homeostasis. Through secretion of insulin and other hormones …
Linker engineering in the context of synthetic protein switches and sensors
A Gräwe, V Stein - Trends in biotechnology, 2021 - cell.com
Linkers play critical roles in the construction of synthetic protein switches and sensors as
they functionally couple a receptor with an actuator. With an increasing number of molecular …
they functionally couple a receptor with an actuator. With an increasing number of molecular …
Visualizing physiological parameters in cells and tissues using genetically encoded indicators for metabolites
A San Martín, R Arce-Molina, C Aburto… - Free Radical Biology …, 2022 - Elsevier
The study of metabolism is undergoing a renaissance. Since the year 2002, over 50
genetically-encoded fluorescent indicators (GEFIs) have been introduced, capable of …
genetically-encoded fluorescent indicators (GEFIs) have been introduced, capable of …