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Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii
Since the mid-1960s, methylotrophic yeast Komagataella phaffii (previously described as
Pichia pastoris) has received increasing scientific attention. The interest for the industrial …
Pichia pastoris) has received increasing scientific attention. The interest for the industrial …
A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production
The methylotrophic yeast Pichia pastoris is garnering interest as a chassis cell factory for the
manufacture of recombinant proteins because it effectively satisfies the requirements of both …
manufacture of recombinant proteins because it effectively satisfies the requirements of both …
Heterologous expression of the novel dimeric antimicrobial peptide LIG in Pichia pastoris
L Zhao, L Li, M Hu, Y Fang, N Dong, A Shan - Journal of Biotechnology, 2024 - Elsevier
The antimicrobial peptide (AMP) LI is a fusion product of antimicrobial peptide LL37
produced by human neutrophils and Indolicidin secreted by bovine neutrophils. LI retained …
produced by human neutrophils and Indolicidin secreted by bovine neutrophils. LI retained …
Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
TM Khlebodarova, NV Bogacheva, AV Zadorozhny… - Microorganisms, 2024 - mdpi.com
In the 1980s, Escherichia coli was the preferred host for heterologous protein expression
owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and …
owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and …
[HTML][HTML] Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism
Komagataella phaffii (aka Pichia pastoris) is a yeast able to grow in methanol as the sole
carbon and energy source. This substrate is converted into formaldehyde, a toxic …
carbon and energy source. This substrate is converted into formaldehyde, a toxic …
Enhancing the expression of chondroitin 4-O-sulfotransferase for one-pot enzymatic synthesis of chondroitin sulfate A
Chondroitin sulfate (CS) stands as a pivotal compound in dietary supplements for
osteoarthritis treatment, propelling significant interest in the biotechnological pursuit of …
osteoarthritis treatment, propelling significant interest in the biotechnological pursuit of …
[HTML][HTML] Advances in Komagataella phaffii Engineering for the Production of Renewable Chemicals and Proteins
The need for a more sustainable society has prompted the development of bio-based
processes to produce fuels, chemicals, and materials in substitution for fossil-based ones. In …
processes to produce fuels, chemicals, and materials in substitution for fossil-based ones. In …
Strategic approaches for designing yeast strains as protein secretion and display platforms
Yeast has been established as a versatile platform for expressing functional molecules,
owing to its well-characterized biology and extensive genetic modification tools. Compared …
owing to its well-characterized biology and extensive genetic modification tools. Compared …
High-Level Expression and Biochemical Characterization of a Maltotetraose Amylase in Pichia pastoris X-33 for Maltotetraose Production
X Wang, M Xu, X Ren, M Li, C Wang, F Yang… - Applied Biochemistry and …, 2024 - Springer
Maltotetraose amylase, which catalyzes the hydrolysis of amylaceous polysaccharides into
maltooligosaccharides with maltotetraose as the main product, is extensively used in the …
maltooligosaccharides with maltotetraose as the main product, is extensively used in the …
Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii
Promoters are crucial elements for engineering microbial production strains used in
bioprocesses. For the increasingly popular chassis Komagataella phaffii (formerly Pichia …
bioprocesses. For the increasingly popular chassis Komagataella phaffii (formerly Pichia …