Highlights•13 C tracer analysis can be used to probe intracellular metabolism in
cells.•Steady state labeling patterns can quantify nutrient contributions.•Steady state labeling …

Interactions with the microbiota influence many aspects of immunity, including immune cell
development, differentiation, and function. Here, we examined the impact of the microbiota …

The composition of the intestinal microbiome varies considerably between individuals and is
correlated with health. Understanding the extent to which, and how, host genetics …

Abstract Changes in mitochondrial morphology are associated with nutrient utilization, but
the precise causalities and the underlying mechanisms remain unknown. Here, using …

Summary mRNA translation is thought to be the most energy-consuming process in the cell.
Translation and energy metabolism are dysregulated in a variety of diseases including …

Activated macrophages switch from oxidative phosphorylation to aerobic glycolysis, similar
to the Warburg effect, presenting a potential therapeutic target in inflammatory disease. The …

The metabolism of endothelial cells during vessel sprouting remains poorly studied. Here
we report that endothelial loss of CPT1A, a rate-limiting enzyme of fatty acid oxidation (FAO) …

Highlights•Tracer experiments, analytics and modeling constitute the 13 C fluxomics
triumvirate.•13 C fluxomics tools differ in complexity, information power and universality.•The …

Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon
in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at …

The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …