Mass-spectrometry based proteomics has evolved as a promising technology over the last
decade and is undergoing a dramatic development in a number of different areas, such as; …

Mass spectrometry (MS)-based blood proteomics is a crucial research focus in identifying
disease biomarkers. Blood serum or plasma is the most commonly used sample for such …

Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes
in a fast and reproducible manner. Here we present the narrow-window data-independent …

Today's highly accurate spectra provided by modern tandem mass spectrometers offer
considerable advantages for the analysis of proteomic samples of increased complexity …

We describe the comprehensive analysis of the yeast proteome in just over one hour of
optimized analysis. We achieve this expedited proteome characterization with improved …

In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass
spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been …

Cell signaling mechanisms often transmit information via posttranslational protein
modifications, most importantly reversible protein phosphorylation. Here we develop and …

Functional genomic strategies have become fundamental for annotating gene function and
regulatory networks. Here, we combined functional genomics with proteomics by quantifying …

Protein ubiquitination is a post-translational modification (PTM) that regulates various
aspects of protein function by different mechanisms. Characterization of ubiquitination has …

The complexity of proteomic instrumentation for LC-MS/MS introduces many possible
sources of variability. Data-dependent sampling of peptides constitutes a stochastic element …