Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Single-photon avalanche diode imagers in biophotonics: review and outlook

C Bruschini, H Homulle, IM Antolovic, S Burri… - Light: Science & …, 2019 - nature.com
Single-photon avalanche diode (SPAD) arrays are solid-state detectors that offer imaging
capabilities at the level of individual photons, with unparalleled photon counting and time …

A guide to fluorescent protein FRET pairs

BT Bajar, ES Wang, S Zhang, MZ Lin, J Chu - Sensors, 2016 - mdpi.com
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Fluorescence lifetime imaging microscopy

B Torrado, B Pannunzio, L Malacrida… - Nature Reviews …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence

MS Panagopoulou, AW Wark, DJS Birch… - Journal of extracellular …, 2020 - Taylor & Francis
Extracellular vesicles (EVs) have numerous potential applications in the field of healthcare
and diagnostics, and research into their biological functions is rapidly increasing. Mainly …

A Time Correlated SPAD Image Sensor in 40-nm CMOS Technology

RK Henderson, N Johnston… - IEEE Journal of Solid …, 2019 - ieeexplore.ieee.org
A pixel single photon avalanche diode (SPAD) time-resolved single photon counting
(TCSPC) image sensor is implemented in STMicroelectronics 40-nm CMOS technology. The …

Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding

M Barroso, MG Monaghan, R Niesner… - Advanced Drug Delivery …, 2023 - Elsevier
Organoid models have been used to address important questions in developmental and
cancer biology, tissue repair, advanced modelling of disease and therapies, among other …

A SPAD-based QVGA image sensor for single-photon counting and quanta imaging

NAW Dutton, I Gyongy, L Parmesan… - … on Electron Devices, 2015 - ieeexplore.ieee.org
A CMOS single-photon avalanche diode (SPAD)-based quarter video graphics array image
sensor with 8-μm pixel pitch and 26.8% fill factor (FF) is presented. The combination of …

Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array

K Samimi, DE Desa, W Lin, K Weiss… - Journal of …, 2023 - spiedigitallibrary.org
Significance Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme
nicotinamide adenine dinucleotide (phosphate)[NAD (P) H] is a popular method to monitor …