Bacterial addiction module toxin Doc inhibits translation elongation through its association with the 30S ribosomal subunit

M Liu, Y Zhang, M Inouye… - Proceedings of the …, 2008 - National Acad Sciences
Bacterial toxin–antitoxin (TA) systems (or “addiction modules”) typically facilitate cell survival
during intervals of stress by inducing a state of reversible growth arrest. However, upon …

SlyD proteins from different species exhibit high prolyl isomerase and chaperone activities

C Scholz, B Eckert, F Hagn, P Schaarschmidt… - Biochemistry, 2006 - ACS Publications
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of
an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C …

Yeast mitochondrial Gln-tRNAGln is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS

M Frechin, B Senger, M Brayé, D Kern… - Genes & …, 2009 - genesdev.cshlp.org
It is impossible to predict which pathway, direct glutaminylation of tRNAGln or tRNA-
dependent transamidation of glutamyl-tRNAGln, generates mitochondrial glutaminyl …

Generation of a genetically encoded, photoactivatable intein for the controlled production of cyclic peptides

JK Böcker, K Friedel, JCJ Matern… - Angewandte …, 2015 - Wiley Online Library
Cyclic peptides are important natural products and hold great promise for the identification of
new bioactive molecules. The split‐intein‐mediated SICLOPPS technology provides a …

N‐terminal chemical protein labeling using the naturally split GOS‐TerL intein

AL Bachmann, HD Mootz - Journal of Peptide Science, 2017 - Wiley Online Library
Chemoselective and regioselective chemical protein labeling is of great importance, yet no
current technique is sufficiently general and simple to perform. Protein trans‐splicing by split …

Two-step method to isolate target recombinant protein from co-purified bacterial contaminant SlyD after immobilised metal affinity chromatography

CB Parsy, CJ Chapman, AC Barnes… - … of Chromatography B, 2007 - Elsevier
As part of a study to purify the internal domain of HER2 (ICD) from recombinant expression,
through metal immobilised affinity chromatography (IMAC), we encountered a contaminant …

A high-performance liquid chromatography method for determining transition metal content in proteins

A Atanassova, R Lam, DB Zamble - Analytical biochemistry, 2004 - Elsevier
Transition metals are common components of cellular proteins and the detailed study of
metalloproteins necessitates the identification and quantification of bound metal ions …

Step** transfer messenger RNA through the ribosome

OV Shpanchenko, MI Zvereva, PV Ivanov… - Journal of Biological …, 2005 - ASBMB
tmRNA (transfer messenger RNA) is a unique molecule used by all bacteria to rescue
stalled ribosomes and to mark unfinished peptides with a specific degradation signal …

RapA, the SWI/SNF Subunit of Escherichia coli RNA Polymerase, Promotes the Release of Nascent RNA from Transcription Complexes

B Yawn, L Zhang, C Mura, MV Sukhodolets - Biochemistry, 2009 - ACS Publications
RapA, a prokaryotic member of the SWI/SNF protein superfamily, is an integral part of the
RNA polymerase transcription complex. RapA's function and catalytic mechanism have …

Highly expressed recombinant SEB for antibody production and development of immunodetection system

R Agrawal, PK Singh, SK Sharma, DV Kamboj… - Indian journal of …, 2012 - Springer
Staphylococcal enterotoxins (SEs) are the second most common causal agents of food
poisoning throughout the world. Staphylococcal enterotoxin B (SEB) is one of the most …