Chemical strategies to engineer hydrogels for cell culture

J Lou, DJ Mooney - Nature Reviews Chemistry, 2022 - nature.com
Two-dimensional and three-dimensional cell culture systems are widely used for biological
studies, and are the basis of the organoid, tissue engineering and organ-on-chip research …

Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels

MR Arkenberg, HD Nguyen, CC Lin - Journal of Materials Chemistry B, 2020 - pubs.rsc.org
In recent years, dynamic,'click'hydrogels have been applied in numerous biomedical
applications. Owing to the mild, cytocompatible, and highly specific reaction kinetics, a …

Designer self‐assembling peptide hydrogels to engineer 3D cell microenvironments for cell constructs formation and precise oncology remodeling in ovarian cancer

Z Yang, H Xu, X Zhao - Advanced Science, 2020 - Wiley Online Library
Designer self‐assembling peptides form the entangled nanofiber networks in hydrogels by
ionic‐complementary self‐assembly. This type of hydrogel has realistic biological and …

Effect of polymeric matrix stiffness on osteogenic differentiation of mesenchymal stem/progenitor cells: concise review

AA El-Rashidy, S El Moshy, IA Radwan, D Rady… - Polymers, 2021 - mdpi.com
Mesenchymal stem/progenitor cells (MSCs) have a multi-differentiation potential into
specialized cell types, with remarkable regenerative and therapeutic results. Several factors …

Dynamic modulation of matrix adhesiveness induces epithelial-to-mesenchymal transition in prostate cancer cells in 3D

M Pol, H Gao, H Zhang, OJ George, JM Fox, X Jia - Biomaterials, 2023 - Elsevier
Synthetic matrices with dynamic presentation of cell guidance cues are needed for the
development of physiologically relevant in vitro tumor models. Towards the goal of …

Matrix adhesiveness regulates myofibroblast differentiation from vocal fold fibroblasts in a bio-orthogonally cross-linked hydrogel

J Song, H Gao, H Zhang, OJ George… - … applied materials & …, 2022 - ACS Publications
Repeated mechanical and chemical insults cause an irreversible alteration of extracellular
matrix (ECM) composition and properties, giving rise to vocal fold scarring that is refractory …

Click-functionalized hydrogel design for mechanobiology investigations

E Hui, JL Sumey, SR Caliari - Molecular systems design & …, 2021 - pubs.rsc.org
The advancement of click-functionalized hydrogels in recent years has coincided with rapid
growth in the fields of mechanobiology, tissue engineering, and regenerative medicine …

Tetrazine-based inverse-electron-demand Diels–Alder reaction: a powerful tool for fabrication and functionalization of polymeric materials

M Arslan, A Degirmenci, R Sanyal, A Sanyal - Polymer Chemistry, 2024 - pubs.rsc.org
The advent of the click chemistry paradigm has profoundly transformed the design of
functional polymeric materials. Among the various reactions in the click chemistry toolkit, the …

Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes

H Gao, M Pol, CA Makara, J Song, H Zhang, X Zou… - Nature …, 2024 - nature.com
Described herein is a protocol for producing a synthetic extracellular matrix that can be
modified in situ during three-dimensional cell culture. The hydrogel platform is established …

Dynamic click hydrogels for xeno‐free culture of induced pluripotent stem cells

MR Arkenberg, NH Dimmitt, HC Johnson… - Advanced …, 2020 - Wiley Online Library
Xeno‐free, chemically defined poly (ethylene glycol)(PEG)‐based hydrogels are being
increasingly used for in vitro culture and differentiation of human induced pluripotent stem …