Infrared difference spectroscopy of proteins: from bands to bonds

VA Lorenz-Fonfria - Chemical reviews, 2020 - ACS Publications
Infrared difference spectroscopy probes vibrational changes of proteins upon their
perturbation. Compared with other spectroscopic methods, it stands out by its sensitivity to …

Photophysics of the blue light using flavin domain

A Lukacs, PJ Tonge, SR Meech - Accounts of chemical research, 2022 - ACS Publications
Conspectus Light activated proteins are at the heart of photobiology and optogenetics, so
there is wide interest in understanding the mechanisms coupling optical excitation to protein …

Electrostatic control of photoisomerization pathways in proteins

MG Romei, CY Lin, II Mathews, SG Boxer - Biophysical Journal, 2020 - cell.com
Adam W. Duster1, Hai Lin2. 1Integrative and Systems Biology, University of Colorado
Denver, Denver, CO, USA, 2Chemistry, University of Colorado Denver, Denver, CO, USA …

Unraveling the photoactivation mechanism of a light-activated adenylyl cyclase using ultrafast spectroscopy coupled with unnatural amino acid mutagenesis

J Tolentino Collado, JN Iuliano, K Pirisi… - ACS chemical …, 2022 - ACS Publications
The hydrogen bonding network that surrounds the flavin in blue light using flavin adenine
dinucleotide (BLUF) photoreceptors plays a crucial role in sensing and communicating the …

Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins

T Fujisawa, S Masuda - Biophysical reviews, 2018 - Springer
Photoreceptor proteins have been used to study how protein conformational changes are
induced by alterations in their environments and how their signals are transmitted to …

Use of noncanonical tyrosine analogues to probe control of radical intermediates during endoperoxide installation by verruculogen synthase (FtmOx1)

CY Lin, AL Muñoz Hernández, TN Laremore… - ACS …, 2022 - ACS Publications
Important bioactive natural products, including prostaglandin H2 and artemisinin, contain
reactive endoperoxides. Known enzymatic pathways for endoperoxide installation require …

Early photocycle of Slr1694 blue-light using flavin photoreceptor unraveled through adiabatic excited-state quantum mechanical/molecular mechanical dynamics

JJ Goings, S Hammes-Schiffer - Journal of the American Chemical …, 2019 - ACS Publications
Blue-light using flavin (BLUF) photoreceptor proteins are essential to many biological
processes and are attractive candidates for use in optogenetics. To understand the …

Photoactivation of the BLUF protein PixD probed by the site-specific incorporation of fluorotyrosine residues

AA Gil, SP Laptenok, JN Iuliano, A Lukacs… - Journal of the …, 2017 - ACS Publications
The flavin chromophore in blue-light-using FAD (BLUF) photoreceptors is surrounded by a
hydrogen bond network that senses and responds to changes in the electronic structure of …

Hydrogen bonding environments in the photocycle process around the flavin chromophore of the AppA-BLUF domain

T Iwata, T Nagai, S Ito, S Osoegawa… - Journal of the …, 2018 - ACS Publications
Three kinds of photochemical reactions are known in flavins as chromophores of
photosensor proteins, reflecting the various catalytic reactions of the flavin in flavoenzymes …

Elucidating the Signal Transduction Mechanism of the Blue-Light-Regulated Photoreceptor YtvA: From Photoactivation to Downstream Regulation

YL He, JT Collado, JN Iuliano… - ACS Chemical …, 2024 - ACS Publications
The blue-light photoreceptor YtvA from Bacillus subtilis has an N-terminal flavin
mononucleotide (FMN)-binding light-oxygen-voltage (LOV) domain that is fused to a C …