Structure and function of retroviral integrase
A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy
of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the …
of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the …
Structural basis of human telomerase recruitment by TPP1-POT1
Telomerase maintains genome stability by extending the 3′ telomeric repeats at eukaryotic
chromosome ends, thereby counterbalancing progressive loss caused by incomplete …
chromosome ends, thereby counterbalancing progressive loss caused by incomplete …
Structural basis of seamless excision and specific targeting by piggyBac transposase
Q Chen, W Luo, RA Veach, AB Hickman… - Nature …, 2020 - nature.com
The piggyBac DNA transposon is used widely in genome engineering applications. Unlike
other transposons, its excision site can be precisely repaired without leaving footprints and it …
other transposons, its excision site can be precisely repaired without leaving footprints and it …
Tools for visualizing and analyzing Fourier space sampling in Cryo-EM
A complete understanding of how an orientation distribution contributes to a cryo-EM
reconstruction remains lacking. It is necessary to begin critically assessing the set of views to …
reconstruction remains lacking. It is necessary to begin critically assessing the set of views to …
IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings
Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad
impacts on gene expression, genome evolution, and the spread of drug-resistance in …
impacts on gene expression, genome evolution, and the spread of drug-resistance in …
Structure of the TnsB transposase-DNA complex of type VK CRISPR-associated transposon
F Tenjo-Castaño, N Sofos, B López-Méndez… - Nature …, 2022 - nature.com
CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opted
CRISPR-Cas systems for RNA-guided transposition. Here we present the 2.4 Å cryo-EM …
CRISPR-Cas systems for RNA-guided transposition. Here we present the 2.4 Å cryo-EM …
Structure of TnsABCD transpososome reveals mechanisms of targeted DNA transposition
Tn7-like transposons are characterized by their ability to insert specifically into host
chromosomes. Recognition of the attachment (att) site by TnsD recruits the TnsABC proteins …
chromosomes. Recognition of the attachment (att) site by TnsD recruits the TnsABC proteins …
Engineered Slee** Beauty transposase redirects transposon integration away from genes
Abstract The Slee** Beauty (SB) transposon system is a popular tool for genome
engineering, but random integration into the genome carries a certain genotoxic risk in …
engineering, but random integration into the genome carries a certain genotoxic risk in …
What could go wrong? A practical guide to single-particle cryo-EM: from biochemistry to atomic models
Cryo-electron microscopy (cryo-EM) has enjoyed explosive recent growth due to
revolutionary advances in hardware and software, resulting in a steady stream of long …
revolutionary advances in hardware and software, resulting in a steady stream of long …
The large bat Helitron DNA transposase forms a compact monomeric assembly that buries and protects its covalently bound 5′-transposon end
Helitrons are widespread eukaryotic DNA transposons that have significantly contributed to
genome variability and evolution, in part because of their distinctive, replicative rolling-circle …
genome variability and evolution, in part because of their distinctive, replicative rolling-circle …