Proteins are the primary effectors of function in biology, and thus, complete knowledge of
their structure and properties is fundamental to deciphering function in basic and …

Analyzing proteins from single cells by tandem mass spectrometry (MS) has recently
become technically feasible. While such analysis has the potential to accurately quantify …

MS-based proteome profiling has become increasingly comprehensive and quantitative, yet
a persistent shortcoming has been the relatively large samples required to achieve an in …

Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific
functional networks underlying biological processes. Here, we present a streamlined …

Microfluidics has become a mature technology, providing an excellent toolbox for the
handling and manipulation of fluid samples, suspended cells, and particles. About 30 years …

Mass spectrometry is unmatched in its versatility for studying practically any aspect of the
proteome. Because the foundations of mass spectrometry-based proteomics are complex …

The recent technological and computational advances in mass spectrometry-based single-
cell proteomics have pushed the boundaries of sensitivity and throughput. However …

We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC,
high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap …

Global quantification of protein abundances in single cells could provide direct information
on cellular phenotypes and complement transcriptomics measurements. However, single …

The inability to make broad, minimally biased measurements of a cell's proteome stands as
a major bottleneck for understanding how gene expression translates into cellular …