Gene expression is a fundamentally stochastic process, with randomness in transcription
and translation leading to cell-to-cell variations in mRNA and protein levels. This variation …

Genetically identical cells exposed to the same environmental conditions can show
significant variation in molecular content and marked differences in phenotypic …

It has long been the dream of biologists to map gene expression at the single-cell level. With
such data one might track heterogeneous cell sub-populations, and infer regulatory …

Background Heterogeneity in single-cell RNA-seq (scRNA-seq) data is driven by multiple
sources, including biological variation in cellular state as well as technical variation …

Praise for the first edition:… superb, beautifully written and organized work that takes an
engineering approach to systems biology. Alon provides nicely written appendices to …

Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic
bacterial populations. However, these molecules often exist in low copy numbers and are …

Single-cell genomics has now made it possible to create a comprehensive atlas of human
cells. At the same time, it has reopened definitions of a cell's identity and of the ways in …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

Individual cells in genetically homogeneous populations have been found to express
different numbers of molecules of specific proteins. We investigated the origins of these …

Genetically identical cells and organisms exhibit remarkable diversity even when they have
identical histories of environmental exposure. Noise, or variation, in the process of gene …