Whole-body and whole-organ clearing and imaging techniques with single-cell resolution: toward organism-level systems biology in mammals
Organism-level systems biology aims to identify, analyze, control and design cellular circuits
in organisms. Many experimental and computational approaches have been developed …
in organisms. Many experimental and computational approaches have been developed …
A survey of methods for 3D histology reconstruction
Histology permits the observation of otherwise invisible structures of the internal topography
of a specimen. Although it enables the investigation of tissues at a cellular level, it is invasive …
of a specimen. Although it enables the investigation of tissues at a cellular level, it is invasive …
Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation
MJC Jordão, R Sankowski, SM Brendecke, Sagar… - Science, 2019 - science.org
INTRODUCTION Under homeostasis, the central nervous system (CNS) hosts microglia
(MG) and CNS-associated macrophages (CAMs). During experimental autoimmune …
(MG) and CNS-associated macrophages (CAMs). During experimental autoimmune …
3D U-Net: learning dense volumetric segmentation from sparse annotation
This paper introduces a network for volumetric segmentation that learns from sparsely
annotated volumetric images. We outline two attractive use cases of this method:(1) In a …
annotated volumetric images. We outline two attractive use cases of this method:(1) In a …
Brain capillary pericytes exert a substantial but slow influence on blood flow
The majority of the brain's vasculature is composed of intricate capillary networks lined by
capillary pericytes. However, it remains unclear whether capillary pericytes influence blood …
capillary pericytes. However, it remains unclear whether capillary pericytes influence blood …
[HTML][HTML] Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
Neuronal cell types are the nodes of neural circuits that determine the flow of information
within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides …
within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides …
A new fate map** system reveals context-dependent random or clonal expansion of microglia
Microglia constitute a highly specialized network of tissue-resident immune cells that is
important for the control of tissue homeostasis and the resolution of diseases of the CNS …
important for the control of tissue homeostasis and the resolution of diseases of the CNS …
Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution
INTRODUCTION Neural circuits across the brain are composed of structures spanning
seven orders of magnitude in size that are assembled from thousands of distinct protein …
seven orders of magnitude in size that are assembled from thousands of distinct protein …
[HTML][HTML] Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis
Systems-level identification and analysis of cellular circuits in the brain will require the
development of whole-brain imaging with single-cell resolution. To this end, we performed …
development of whole-brain imaging with single-cell resolution. To this end, we performed …
Inefficient clearance of myelin debris by microglia impairs remyelinating processes
A Lampron, A Larochelle, N Laflamme… - Journal of Experimental …, 2015 - rupress.org
An imbalance between remyelinating and demyelinating rates underlies degenerative
processes in demyelinating diseases such as multiple sclerosis. An optimal therapeutic …
processes in demyelinating diseases such as multiple sclerosis. An optimal therapeutic …