Gene expression in bacteria relies on promoter recognition by the DNA-dependent RNA
polymerase and subsequent transcription initiation. Bacterial cells are able to tune their …

Bacteria use a variety of mechanisms to direct RNA polymerase to specific promoters in
order to activate transcription in response to growth signals or environmental cues …

Initiation of RNA synthesis from DNA templates by RNA polymerase (RNAP) is a multi-step
process, in which initial recognition of promoter DNA by RNAP triggers a series of …

Transcription initiation is a highly regulated step of gene expression. Here, we discuss the
series of large conformational changes set in motion by initial specific binding of bacterial …

Transcription initiation requires formation of the open promoter complex (RPo). To generate
RPo, RNA polymerase (RNAP) unwinds the DNA duplex to form the transcription bubble …

Early work identified two promoter regions, the− 10 and− 35 elements, that interact
sequence specifically with bacterial RNA polymerase (RNAP). However, we now know that …

Background Adenosine triphosphate (ATP) is the main energy carrier in living organisms,
critical for metabolism and essential physiological processes. In humans, abnormal …

Abstract Information theory was used to build a promoter model that accounts for the− 10,
the− 35 and the uncertainty of the gap between them on a common scale. Helical face …

RbpA and CarD are essential transcription regulators in mycobacteria. Mechanistic analyses
of promoter open complex (RPo) formation establish that RbpA and CarD cooperatively …

Bacterial RNA polymerase is composed of a core of subunits (β β′, α1, α2, ω), which have
RNA synthesizing activity, and a specificity factor (σ), which identifies the start of transcription …