Computational coherent Raman scattering imaging: breaking physical barriers by fusion of advanced instrumentation and data science

H Lin, JX Cheng - eLight, 2023‏ - Springer
Coherent Raman scattering (CRS) microscopy is a chemical imaging modality that provides
contrast based on intrinsic biomolecular vibrations. To date, endeavors on instrumentation …

Recent Progress in Deep Learning for Improving Coherent Anti‐Stokes Raman Scattering Microscopy

B Yao, F Lin, Z Luo, Q Chen, D Lin… - Laser & Photonics …, 2024‏ - Wiley Online Library
Abstract Coherent anti‐Stokes Raman scattering (CARS) microscopy is a powerful label‐
free imaging technique that leverages biomolecular vibrations and is widely used in different …

Full-spectrum CARS microscopy of cells and tissues with ultrashort white-light continuum pulses

F Vernuccio, R Vanna, C Ceconello… - The Journal of …, 2023‏ - ACS Publications
Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging nonlinear
vibrational imaging technique that delivers label-free chemical maps of cells and tissues. In …

Pulse-picking multimodal nonlinear optical microscopy

MG Clark, GA Gonzalez, C Zhang - Analytical Chemistry, 2022‏ - ACS Publications
Nonlinear optical microscopy techniques can map chemical compositions in biological
samples in a label-free manner. Commonly used nonlinear optical processes for imaging …

Raman micro-spectroscopy reveals the spatial distribution of fumarate in cells and tissues

M Kamp, J Surmacki, M Segarra Mondejar… - Nature …, 2024‏ - nature.com
Aberrantly accumulated metabolites elicit intra-and inter-cellular pro-oncogenic cascades,
yet current measurement methods require sample perturbation/disruption and lack spatio …

[HTML][HTML] Removing non-resonant background of CARS signal with generative adversarial network

Z Luo, X Xu, D Lin, J Qu, F Lin, J Li - Applied Physics Letters, 2024‏ - pubs.aip.org
Coherent anti-Stokes Raman scattering (CARS) microscopy requires the removal of non-
resonant background (NRB) to ensure spectral accuracy and quality. This study introduces a …

Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal

A De la Cadena, J Park, KF Tehrani… - Biomedical Optics …, 2024‏ - opg.optica.org
Nonlinear microscopy encompasses several imaging techniques that leverage laser
technology to probe intrinsic molecules of biological specimens. These native molecules …

Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions

B Talone, A Bresci, F Manetti, F Vernuccio… - … in Bioengineering and …, 2022‏ - frontiersin.org
Bone tissue features a complex microarchitecture and biomolecular composition, which
determine biomechanical properties. In addition to state-of-the-art technologies, innovative …

[HTML][HTML] Dual picosecond fast tunable optical parametric amplifier laser system for wide-field nonlinear optical microscopy

F Vernuccio, A Benachir, EM Fantuzzi, B Morel, S Bux… - APL Photonics, 2024‏ - pubs.aip.org
In the last decades, nonlinear optical microscopy techniques, such as two-Photon Excited
Fluorescence (2PEF), Sum Frequency Generation (SFG), and Hyperspectral Coherent Anti …

Estimation of biological variance in coherent Raman microscopy data of two cell lines using chemometrics

R Junjuri, M Calvarese, MS Vafaeinezhad, F Vernuccio… - Analyst, 2024‏ - pubs.rsc.org
Broadband Coherent Anti-Stokes Raman Scattering (BCARS) is a valuable spectroscopic
imaging tool for visualizing cellular structures and lipid distributions in biomedical …