Learning epistasis and residue coevolution patterns: Current trends and future perspectives for advancing enzyme engineering

M Wittmund, F Cadet, MD Davari - ACS catalysis, 2022‏ - ACS Publications
Engineering proteins and enzymes with the desired functionality has broad applications in
molecular biology, biotechnology, biomedical sciences, health, and medicine. The vastness …

Generative and interpretable machine learning for aptamer design and analysis of in vitro sequence selection

A Di Gioacchino, J Procyk, M Molari… - PLoS computational …, 2022‏ - journals.plos.org
Selection protocols such as SELEX, where molecules are selected over multiple rounds for
their ability to bind to a target of interest, are popular methods for obtaining binders for …

ACIDES: on-line monitoring of forward genetic screens for protein engineering

T Nemoto, T Ocari, A Planul, M Tekinsoy, EA Zin… - Nature …, 2023‏ - nature.com
Forward genetic screens of mutated variants are a versatile strategy for protein engineering
and investigation, which has been successfully applied to various studies like directed …

Inference of annealed protein fitness landscapes with AnnealDCA

L Sesta, A Pagnani… - PLOS Computational …, 2024‏ - journals.plos.org
The design of proteins with specific tasks is a major challenge in molecular biology with
important diagnostic and therapeutic applications. High-throughput screening methods have …

In-silico monitoring of directed evolution convergence to unveil best performing variants with credibility score

T Nemoto, T Ocari, A Planul, M Tekinsoy, EA Zin… - bioRxiv, 2023‏ - biorxiv.org
Directed evolution (DE) is a versatile protein-engineering strategy applicable to proteins like
enzymes, antibodies, or viral vectors. Starting from a library containing billions of random …

Protein evolution and data-driven sequence landscapes

M Bisardi - 2023‏ - theses.hal.science
Thanks to the explosion of available protein sequence data, driven by next-generation
sequencing, unsupervised machine learning models can be harnessed to learn protein …

[PDF][PDF] Unsupervised inference methods for protein sequence data

L Sesta - 2023‏ - tesidottorato.depositolegale.it
Recent years have seen an explosion of availability of protein sequence data. However, the
vast majority of these data are unlabeled, that is, the sequences are not accompanied by …

[HTML][HTML] Uncursing winner's curse: on-line monitoring of directed evolution convergence

T Nemoto, T Ocari, A Planul, M Tekinsoy, EA Zin… - 2023‏ - europepmc.org
Directed evolution (DE) is a versatile protein-engineering strategy, successfully applied to a
range of proteins, including enzymes, antibodies, and viral vectors. However, DE can be …

Computational Analysis & Design of Biopolymers

J Procyk - 2022‏ - search.proquest.com
Biopolymers perform the majority of essential functions necessary for life. From a small
amount of components emerges considerable complexity in both structure and function. The …