(S) Pinning down protein interactions by NMR
Protein molecules are highly diverse communication platforms and their interaction
repertoire stretches from atoms over small molecules such as sugars and lipids to …
repertoire stretches from atoms over small molecules such as sugars and lipids to …
[HTML][HTML] Intracellular glutathione pools are heterogeneously concentrated
Glutathione is present in millimolar concentrations in the cell, but its relative distribution
among cellular compartments remains elusive. We have chosen the endoplasmic reticulum …
among cellular compartments remains elusive. We have chosen the endoplasmic reticulum …
Characterizing rapid, activity‐linked conformational transitions in proteins via sub‐second hydrogen deuterium exchange mass spectrometry
This review outlines the application of time‐resolved electrospray ionization mass
spectrometry (TRESI‐MS) and hydrogen‐deuterium exchange (HDX) to study rapid, activity …
spectrometry (TRESI‐MS) and hydrogen‐deuterium exchange (HDX) to study rapid, activity …
Mycoredoxin‐1 is one of the missing links in the oxidative stress defence mechanism of M ycobacteria
K Van Laer, L Buts, N Foloppe… - Molecular …, 2012 - Wiley Online Library
To survive hostile conditions, the bacterial pathogen M ycobacterium tuberculosis produces
millimolar concentrations of mycothiol as a redox buffer against oxidative stress. The …
millimolar concentrations of mycothiol as a redox buffer against oxidative stress. The …
Protein dielectric constants determined from NMR chemical shift perturbations
Understanding the connection between protein structure and function requires a quantitative
understanding of electrostatic effects. Structure-based electrostatic calculations are essential …
understanding of electrostatic effects. Structure-based electrostatic calculations are essential …
Iron–Sulfur Cluster Binding by Mitochondrial Monothiol Glutaredoxin-1 of Trypanosoma brucei: Molecular Basis of Iron–Sulfur Cluster Coordination and Relevance …
Abstract Aims: Monothiol glutaredoxins (1-C-Grxs) are small proteins linked to the cellular
iron and redox metabolism. Trypanosoma brucei brucei, model organism for human African …
iron and redox metabolism. Trypanosoma brucei brucei, model organism for human African …
The pKa Value and Accessibility of Cysteine Residues Are Key Determinants for Protein Substrate Discrimination by Glutaredoxin
The enzyme glutaredoxin catalyzes glutathione exchange, but little is known about its
interaction with protein substrates. Very different proteins are substrates in vitro, and the …
interaction with protein substrates. Very different proteins are substrates in vitro, and the …
[HTML][HTML] The dithiol mechanism of class I glutaredoxins promotes specificity for glutathione as a reducing agent
L Lang, P Reinert, C Diaz, M Deponte - Redox Biology, 2024 - Elsevier
Class I glutaredoxins reversibly reduce glutathione-and nonglutathione disulfides with the
help of reduced glutathione (GSH) using either a monothiol mechanism or a dithiol …
help of reduced glutathione (GSH) using either a monothiol mechanism or a dithiol …
[PDF][PDF] Conformer selection and intensified dynamics during catalytic turnover in chymotrypsin
During catalytic turnover, enzymes undergo thermally driven conformational fluctuations
(dynamics) that are directly linked to catalytic efficiency.[1–3] In broad terms, this link exists …
(dynamics) that are directly linked to catalytic efficiency.[1–3] In broad terms, this link exists …
Kinetic studies reveal a key role of a redox-active glutaredoxin in the evolution of the thiol-redox metabolism of trypanosomatid parasites
Trypanosomes are flagellated protozoan parasites (kinetoplastids) that have a unique redox
metabolism based on the small dithiol trypanothione (T (SH) 2). Although GSH may still play …
metabolism based on the small dithiol trypanothione (T (SH) 2). Although GSH may still play …