Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Killing mechanisms of chimeric antigen receptor (CAR) T cells
MR Benmebarek, CH Karches, BL Cadilha… - International journal of …, 2019 - mdpi.com
Effective adoptive T cell therapy (ACT) comprises the killing of cancer cells through the
therapeutic use of transferred T cells. One of the main ACT approaches is chimeric antigen …
therapeutic use of transferred T cells. One of the main ACT approaches is chimeric antigen …
Engineering broad-spectrum bacterial blight resistance by simultaneously disrupting variable TALE-binding elements of multiple susceptibility genes in rice
Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice, employs
the transcription activator-like effectors (TALEs) to induce the expression of the OsSWEET …
the transcription activator-like effectors (TALEs) to induce the expression of the OsSWEET …
Genome-editing technologies for gene and cell therapy
ML Maeder, CA Gersbach - Molecular therapy, 2016 - cell.com
Gene therapy has historically been defined as the addition of new genes to human cells.
However, the recent advent of genome-editing technologies has enabled a new paradigm in …
However, the recent advent of genome-editing technologies has enabled a new paradigm in …
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …
comprise a powerful class of tools that are redefining the boundaries of biological research …
A guide to genome engineering with programmable nucleases
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
Precision mitochondrial DNA editing with high-fidelity DddA-derived base editors
Bacterial toxin DddA-derived cytosine base editors (DdCBEs)—composed of split DddAtox
(a cytosine deaminase specific to double-stranded DNA), custom-designed TALE …
(a cytosine deaminase specific to double-stranded DNA), custom-designed TALE …
Strand-selective base editing of human mitochondrial DNA using mitoBEs
A number of mitochondrial diseases in humans are caused by point mutations that could be
corrected by base editors, but delivery of CRISPR guide RNAs into the mitochondria is …
corrected by base editors, but delivery of CRISPR guide RNAs into the mitochondria is …
[HTML][HTML] TALE and TALEN genome editing technologies
S Becker, J Boch - Gene and Genome Editing, 2021 - Elsevier
TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …
editing revolution. Their application in multiple species brought targeted mutagenesis to the …
CRISPR/Cas9 for genome editing: progress, implications and challenges
F Zhang, Y Wen, X Guo - Human molecular genetics, 2014 - academic.oup.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling …
(Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling …