Genome-scale metabolic models (GEMs) computationally describe gene-protein-reaction
associations for entire metabolic genes in an organism, and can be simulated to predict …

The construction of high-performance microbial cell factories (MCFs) is the centerpiece of
biomanufacturing. However, the complex metabolic regulatory network of microorganisms …

Genome-scale metabolic models (GEMs) represent extensive knowledgebases that provide
a platform for model simulations and integrative analysis of omics data. This study …

Microorganisms rarely live isolated in their natural environments but rather function in
consolidated and socializing communities. Despite the growing availability of high …

Malonyl-coenzyme A is an important precursor metabolite for the biosynthesis of
polyketides, flavonoids and biofuels. However, malonyl-CoA naturally synthesized in …

Yeasts have been widely used for production of bread, beer and wine, as well as for
production of bioethanol, but they have also been designed as cell factories to produce …

Background Systems biology projects and omics technologies have led to a growing
number of biochemical pathway models and reconstructions. However, the majority of these …

The metabolism of microorganisms is regulated through two main mechanisms: changes of
enzyme capacities as a consequence of gene expression modulation (“hierarchical control”) …

Background Constraint-based analysis of genome-scale metabolic models typically relies
upon maximisation of a cellular objective function such as the rate or efficiency of biomass …

We present an experimental and computational pipeline for the generation of kinetic models
of metabolism, and demonstrate its application to glycolysis in Saccharomyces cerevisiae …