Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing

L Zhao, H Zhang, MV Kohnen, KVSK Prasad… - Frontiers in …, 2019 - frontiersin.org
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …

The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding

X Shi, S Cao, X Wang, S Huang, Y Wang… - Horticulture …, 2023 - academic.oup.com
Grapevine is one of the most economically important crops worldwide. However, the
previous versions of the grapevine reference genome tipically consist of thousands of …

A bioinformatics guide to plant microbiome analysis

R Lucaciu, C Pelikan, SM Gerner, C Zioutis… - Frontiers in plant …, 2019 - frontiersin.org
Recent evidence for intimate relationship of plants with their microbiota shows that plants
host individual and diverse microbial communities that are essential for their survival …

The population genetics of structural variants in grapevine domestication

Y Zhou, A Minio, M Massonnet, E Solares, Y Lv… - Nature plants, 2019 - nature.com
Structural variants (SVs) are a largely unexplored feature of plant genomes. Little is known
about the type and size of SVs, their distribution among individuals and, especially, their …

The genetic basis of sex determination in grapes

M Massonnet, N Cochetel, A Minio, AM Vondras… - Nature …, 2020 - nature.com
It remains a major challenge to identify the genes and mutations that lead to plant sexual
differentiation. Here, we study the structure and evolution of the sex-determining region …

A high-resolution single-molecule sequencing-based Arabidopsis transcriptome using novel methods of Iso-seq analysis

R Zhang, R Kuo, M Coulter, CPG Calixto, JC Entizne… - Genome biology, 2022 - Springer
Background Accurate and comprehensive annotation of transcript sequences is essential for
transcript quantification and differential gene and transcript expression analysis. Single …

The genomic diversification of grapevine clones

AM Vondras, A Minio, B Blanco-Ulate… - BMC genomics, 2019 - Springer
Background Vegetatively propagated clones accumulate somatic mutations. The purpose of
this study was to better appreciate clone diversity and involved defining the nature of …

The hidden world within plants: metatranscriptomics unveils the complexity of wood microbiomes

L Nerva, JF Garcia, F Favaretto… - Journal of …, 2022 - academic.oup.com
The importance of plants as complex entities influenced by genomes of the associated
microorganisms is now seen as a new source of variability for a more sustainable …

Multiple independent recombinations led to hermaphroditism in grapevine

C Zou, M Massonnet, A Minio, S Patel, V Llaca… - Proceedings of the …, 2021 - pnas.org
Hermaphroditic (perfect) flowers were a key trait in grapevine domestication, enabling a
drastic increase in yields due to the efficiency of self-pollination in the domesticated …

Diploid genome assembly of the wine grape Carménère

A Minio, M Massonnet… - G3: Genes …, 2019 - academic.oup.com
In this genome report, we describe the sequencing and annotation of the genome of the
wine grape Carménère (clone 02, VCR-702). Long considered extinct, this old French wine …