Upstream improvements have led to significant advances in the productivity of biomolecules
and bioparticles. Today, downstream processes are the bottleneck in the production of some …

The actual biotechnology industry demands fast and economic upstream and downstream
processes to purify biomolecules. In this context, different purification techniques, that offer …

Nonionic surfactants display different behaviors in aqueous solution, depending on their
concentration and the presence of small amounts of salts and/or ionic surfactants. Here …

The enhanced green fluorescent protein (EGFP) is widely used as a marker in life science.
Currently, purifications of the EGFP mostly involve chromatographic methods, which are …

Aqueous two-phase systems have frequently been utilized as an efficient bioseparation tool
in the downstream processing of different biomolecules. The poly (ethyleneglycol)/poly …

Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker;
however, its widespread use is limited by its purification costs. Alternative low-cost …

Green fluorescent protein (GFP) is a useful biomolecule in biotechnology; however, its price
makes its widespread application prohibitive. To overcome this issue, recently, the use of …

The production of recombinant biopharmaceuticals is highly dependent of a proper choice of
the downstream processing stages. Particularly, the purification that must ensure that all the …

Monoclonal antibodies (mAbs) are currently the most important class of recombinant protein
therapeutics in the biotechnological and biopharmaceutical industry with more than 250 …

Liquid–liquid extraction for aqueous two-phase micellar systems (ATPMS) is a promising
technique that can either replace or be used as a complementary process to more typical …