Trends in develo** one-pot CRISPR diagnostics strategies

L Chen, M Hu, X Zhou - Trends in Biotechnology, 2024 - cell.com
The integration of nucleic acid amplification (NAA) with the CRISPR detection system has
led to significant advancements and opportunities for development in molecular diagnostics …

CRISPR for companion diagnostics in low-resource settings

Q Xu, Q Xu, C Lyon, TY Hu - Lab on a Chip, 2024 - pubs.rsc.org
New point-of-care tests (POCTs), which are especially useful in low-resource settings, are
needed to expand screening capacity for diseases that cause significant mortality …

New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity

AM Molina Vargas, S Sinha, R Osborn… - Nucleic acids …, 2024 - academic.oup.com
An increasingly pressing need for clinical diagnostics has required the development of novel
nucleic acid-based detection technologies that are sensitive, fast, and inexpensive, and that …

Recent advances in CRISPR-based biosensors for point-of-care pathogen detection

N Mohammad, SS Katkam, Q Wei - The CRISPR Journal, 2022 - liebertpub.com
Infectious pathogens are pressing concerns due to their heavy toll on global health and
socioeconomic infrastructure. Rapid, sensitive, and specific pathogen detection methods are …

Agarose hydrogel-boosted one-tube RPA-CRISPR/Cas12a assay for robust point-of-care detection of zoonotic nematode Anisakis

L Zhao, H Wang, X Chen, L Wang… - Journal of Agricultural …, 2024 - ACS Publications
Rapid and accurate detection of the zoonotic nematode Anisakis is poised to control its
epidemic. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas …

Advancements in CRISPR-Diagnostic Techniques for Rapid on-site Monitoring of Environmental Virus

Z Deng, W Liao, L Bu, J Li, J Li, L Wang, A Yuan… - TrAC Trends in …, 2024 - Elsevier
Highly sensitive and rapid environmental surveillance is crucial due to the significant role of
various environmental media in transmitting multiple viruses. Specifically, when examining …

New design strategies for ultra-specific CRISPR-Cas13a-based RNA-diagnostic tools with single-nucleotide mismatch sensitivity

AMM Vargas, R Osborn, S Sinha, PR Arantes, A Patel… - bioRxiv, 2023 - biorxiv.org
The pressing need for clinical diagnostics has required the development of novel nucleic
acid-based detection technologies that are sensitive, fast, and inexpensive, and that can be …

High-Throughput and Integrated CRISPR/Cas12a-Based Molecular Diagnosis Using a Deep Learning Enabled Microfluidic System

L Zhang, H Wang, S Yang, J Liu, J Li, Y Lu, J Cheng… - ACS …, 2024 - ACS Publications
CRISPR/Cas-based molecular diagnosis demonstrates potent potential for sensitive and
rapid pathogen detection, notably in SARS-CoV-2 diagnosis and mutation tracking. Yet, a …

Multiplexed Detection Strategies for Biosensors Based on the CRISPR-Cas System

C Wei, X Lei, S Yu - ACS Synthetic Biology, 2024 - ACS Publications
A growing number of applications require simultaneous detection of multiplexed nucleic acid
targets in a single reaction, which enables higher information density in combination with …

RPA-CRISPR/Cas12a assay for the diagnosis of bovine Anaplasma marginale infection

A Sutipatanasomboon, J Wongsantichon, S Sakdee… - Scientific Reports, 2024 - nature.com
Anaplasma marginale infection is one of the most common tick-borne diseases, causing a
substantial loss in the beef and dairy production industries. Once infected, the pathogen …