CRISPR-Cas9-mediated homology-directed repair for precise gene editing
CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating
precise site-specific DNA insertions, deletions, and substitutions. These precise edits are …
precise site-specific DNA insertions, deletions, and substitutions. These precise edits are …
Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function
INTRODUCTION Recently, a special class of RNAs has excited researchers and triggered
hundreds of now-published studies. Known as circular RNAs (circRNAs), these RNAs are …
hundreds of now-published studies. Known as circular RNAs (circRNAs), these RNAs are …
Generating genetically modified mice using CRISPR/Cas-mediated genome engineering
Mice with specific gene modifications are valuable tools for studying development and
disease. Traditional gene targeting in mice using embryonic stem (ES) cells, although …
disease. Traditional gene targeting in mice using embryonic stem (ES) cells, although …
[HTML][HTML] Dendritic function of tau mediates amyloid-β toxicity in Alzheimer's disease mouse models
Alzheimer's disease (AD) is characterized by amyloid-β (Aβ) and tau deposition in brain. It
has emerged that Aβ toxicity is tau dependent, although mechanistically this link remains …
has emerged that Aβ toxicity is tau dependent, although mechanistically this link remains …
Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
Abstract Background The CRISPR/Cas9 system is increasingly used for gene inactivation in
mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less …
mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less …
Ubiquitination-deficient mutations in human Piwi cause male infertility by impairing histone-to-protamine exchange during spermiogenesis
LT Gou, JY Kang, P Dai, X Wang, F Li, S Zhao… - Cell, 2017 - cell.com
Genetic studies have elucidated critical roles of Piwi proteins in germline development in
animals, but whether Piwi is an actual disease gene in human infertility remains unknown …
animals, but whether Piwi is an actual disease gene in human infertility remains unknown …
[HTML][HTML] Animal models reveal role for tau phosphorylation in human disease
Many proteins that are implicated in human disease are posttranslationally modified. This
includes the microtubule-associated protein tau that is deposited in a hyperphosphorylated …
includes the microtubule-associated protein tau that is deposited in a hyperphosphorylated …
Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer's mice
A Ittner, SW Chua, J Bertz, A Volkerling… - Science, 2016 - science.org
Amyloid-β (Aβ) toxicity in Alzheimer's disease (AD) is considered to be mediated by
phosphorylated tau protein. In contrast, we found that, at least in early disease, site-specific …
phosphorylated tau protein. In contrast, we found that, at least in early disease, site-specific …
Mutations in disordered regions can cause disease by creating dileucine motifs
Many disease-causing missense mutations affect intrinsically disordered regions (IDRs) of
proteins, but the molecular mechanism of their pathogenicity is enigmatic. Here, we employ …
proteins, but the molecular mechanism of their pathogenicity is enigmatic. Here, we employ …
Parkinsonism and impaired axonal transport in a mouse model of frontotemporal dementia
Frontotemporal dementia (FTD) is characterized by cognitive and behavioral changes and,
in a significant subset of patients, Parkinsonism. Histopathologically, FTD frequently …
in a significant subset of patients, Parkinsonism. Histopathologically, FTD frequently …