Gene expression is a fundamentally stochastic process, with randomness in transcription
and translation leading to cell-to-cell variations in mRNA and protein levels. This variation …

Synthetic biology is a research field that combines the investigative nature of biology with
the constructive nature of engineering. Efforts in synthetic biology have largely focused on …

Despite extensive research, our understanding of the rules according to which cis-regulatory
sequences are converted into gene expression is limited. We devised a method for …

Clonal populations of microbial cells often show a high degree of phenotypic variability
under homogeneous conditions. Stochastic fluctuations in the cellular components that …

Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within
transcriptional networks. Here, we present a synthetic framework for systematically …

Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically,
precise control of key pathway enzymes (heterologous or native) can help maximize product …

A dynamic range of well‐controlled constitutive and tunable promoters are essential for
metabolic engineering and synthetic biology applications in all host organisms. Here, we …

Engineering artificial gene networks from modular components is a major goal of synthetic
biology. However, the construction of gene networks with predictable functions remains …

Metabolic pathway engineering in the yeast Saccharomyces cerevisiae leads to improved
production of a wide range of compounds, ranging from ethanol (from biomass) to natural …

As the ability to analyze individual cells in microbial populations expands, it is becoming
apparent that isogenic microbial populations contain substantial cell-to-cell differences in …