Control strategies of ice nucleation, growth, and recrystallization for cryopreservation

M Lin, H Cao, J Li - Acta Biomaterialia, 2023 - Elsevier
The cryopreservation of biomaterials is fundamental to modern biotechnology and
biomedicine, but the biggest challenge is the formation of ice, resulting in fatal cryoinjury to …

Macromolecular cryoprotectants for the preservation of mammalian cell culture: lessons from crowding, overview and perspectives

M Gore, A Narvekar, A Bhagwat, R Jain… - Journal of Materials …, 2022 - pubs.rsc.org
Cryopreservation is a process used for the storage of mammalian cells at a very low
temperature, in a state of 'suspended animation.'Highly effective and safe macromolecular …

Deep eutectic solvents as cryoprotective agents for mammalian cells

SJ Bryant, MN Awad, A Elbourne… - Journal of Materials …, 2022 - pubs.rsc.org
Cryopreservation has facilitated numerous breakthroughs including assisted reproductive
technology, stem cell therapies, and species preservation. Successful cryopreservation …

Advanced cryopreservation as an emergent and convergent technological platform

E Brister, PB Thompson, SM Wolf, JC Bischof - Technology in Society, 2024 - Elsevier
Advanced cryopreservation technologies have the potential to transform organ transplants,
biomedical research, food storage, aquaculture, biodiversity repositories, ecological …

Sulfoxide-functional trehalose enhances DMSO-free cryopreservation of mammalian cells

M Liu, L Liang, C Yu, B Guo, H Zhang, F Yao… - Chemical Engineering …, 2024 - Elsevier
Cell cryopreservation is crucial for biomedicine, and the addition of cryoprotectants (CPAs)
to protect cells from cryoinjuries is essential at low temperatures. Trehalose (Tre) has been …

Natural cryoprotective and cytoprotective agents in cryopreservation: A focus on melatonin

G Marcantonini, D Bartolini, L Zatini, S Costa… - Molecules, 2022 - mdpi.com
Cryoprotective and cytoprotective agents (Cytoprotective Agents) are fundamental
components of the cryopreservation process. This review presents the essentials of the …

Antifreeze proteins: Novel applications and navigation towards their clinical application in cryobanking

MD Ekpo, J **e, Y Hu, X Liu, F Liu, J **ang… - International Journal of …, 2022 - mdpi.com
Antifreeze proteins (AFPs) or thermal hysteresis (TH) proteins are biomolecular gifts of
nature to sustain life in extremely cold environments. This family of peptides, glycopeptides …

Cryopreservation: a comprehensive overview, challenges, and future perspectives

A Parihar, A Kumar, U Panda, R Khan… - Advanced …, 2023 - Wiley Online Library
Cryopreservation is the most prevalent method of long‐term cell preservation. Effective cell
cryopreservation depends on freezing, adequate storage, and correct thawing techniques …

Principles and protocols for post-cryopreservation quality evaluation of stem cells in novel biomedicine

J **e, MD Ekpo, J **ao, H Zhao, X Bai… - Frontiers in …, 2022 - frontiersin.org
Stem cell therapy is a thriving topic of interest among researchers and clinicians due to
evidence of its effectiveness and promising therapeutic advantage in numerous disease …

The role of cryoprotective agents in liposome stabilization and preservation

GF Boafo, KT Magar, MD Ekpo, W Qian, S Tan… - International journal of …, 2022 - mdpi.com
To improve liposomes' usage as drug delivery vehicles, cryoprotectants can be utilized to
prevent constituent leakage and liposome instability. Cryoprotective agents (CPAs) or …