Agrobacterium-mediated plant transformation: biology and applications

HH Hwang, M Yu, EM Lai - The arabidopsis book, 2017 - pmc.ncbi.nlm.nih.gov
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …

[HTML][HTML] Current progress and challenges in crop genetic transformation

RB Anjanappa, W Gruissem - Journal of Plant Physiology, 2021 - Elsevier
Plant transformation remains the most sought-after technology for functional genomics and
crop genetic improvement, especially for introducing specific new traits and to modify or …

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

HL **ng, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …

Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

NA Abdallah, H Elsharawy, HA Abulela… - GM crops & …, 2025 - Taylor & Francis
Genome editing tools have rapidly been adopted by plant scientists for crop improvement.
Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful …

[BOG][B] Genomes 5

TA Brown - 2023 - taylorfrancis.com
Genomes 5 has been completely revised and updated. It is a thoroughly modern textbook
about genomes and how they are investigated. As with previous Genomes editions …

Engineering the plastid and mitochondrial genomes of flowering plants

P Maliga - Nature Plants, 2022 - nature.com
Engineering the plastid genome based on homologous recombination is well developed in
a few model species. Homologous recombination is also the rule in mitochondria, but …

A highly efficient agrobacterium-mediated method for transient gene expression and functional studies in multiple plant species

Y Zhang, M Chen, B Siemiatkowska, MR Toleco… - Plant …, 2020 - cell.com
Although the use of stable transformation technology has led to great insight into gene
function, its application in high-throughput studies remains arduous. Agro-infiltration have …

[HTML][HTML] Biotechnological insights on the expression and production of antimicrobial peptides in plants

B Shanmugaraj, CJI Bulaon, A Malla, W Phoolcharoen - Molecules, 2021 - mdpi.com
The emergence of drug-resistant pathogens poses a serious critical threat to global public
health and requires immediate action. Antimicrobial peptides (AMPs) are a class of short …

Breakthrough in CRISPR/Cas system: Current and future directions and challenges

A Ali, MM Zafar, Z Farooq, SR Ahmed… - Biotechnology …, 2023 - Wiley Online Library
Targeted genome editing (GE) technology has brought a significant revolution in fictional
genomic research and given hope to plant scientists to develop desirable varieties. This …

An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)

S Hayta, MA Smedley, SU Demir, R Blundell… - Plant Methods, 2019 - Springer
Background Despite wheat being a worldwide staple, it is still considered the most difficult to
transform out of the main cereal crops. Therefore, for the wheat research community, a freely …