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Agrobacterium-mediated plant transformation: biology and applications
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …
[HTML][HTML] Current progress and challenges in crop genetic transformation
Plant transformation remains the most sought-after technology for functional genomics and
crop genetic improvement, especially for introducing specific new traits and to modify or …
crop genetic improvement, especially for introducing specific new traits and to modify or …
A CRISPR/Cas9 toolkit for multiplex genome editing in plants
HL **ng, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat
Genome editing tools have rapidly been adopted by plant scientists for crop improvement.
Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful …
Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful …
Engineering the plastid and mitochondrial genomes of flowering plants
P Maliga - Nature Plants, 2022 - nature.com
Engineering the plastid genome based on homologous recombination is well developed in
a few model species. Homologous recombination is also the rule in mitochondria, but …
a few model species. Homologous recombination is also the rule in mitochondria, but …
A highly efficient agrobacterium-mediated method for transient gene expression and functional studies in multiple plant species
Although the use of stable transformation technology has led to great insight into gene
function, its application in high-throughput studies remains arduous. Agro-infiltration have …
function, its application in high-throughput studies remains arduous. Agro-infiltration have …
[HTML][HTML] Biotechnological insights on the expression and production of antimicrobial peptides in plants
The emergence of drug-resistant pathogens poses a serious critical threat to global public
health and requires immediate action. Antimicrobial peptides (AMPs) are a class of short …
health and requires immediate action. Antimicrobial peptides (AMPs) are a class of short …
Breakthrough in CRISPR/Cas system: Current and future directions and challenges
Targeted genome editing (GE) technology has brought a significant revolution in fictional
genomic research and given hope to plant scientists to develop desirable varieties. This …
genomic research and given hope to plant scientists to develop desirable varieties. This …
An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)
S Hayta, MA Smedley, SU Demir, R Blundell… - Plant Methods, 2019 - Springer
Background Despite wheat being a worldwide staple, it is still considered the most difficult to
transform out of the main cereal crops. Therefore, for the wheat research community, a freely …
transform out of the main cereal crops. Therefore, for the wheat research community, a freely …