Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications

Z Li, Y Jiang, FP Guengerich, L Ma, S Li… - Journal of Biological …, 2020 - ASBMB
Cytochrome P450 enzymes (P450s) are broadly distributed among living organisms and
play crucial roles in natural product biosynthesis, degradation of xenobiotics, steroid …

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

N Kumar, J He, JF Rusling - Chemical Society Reviews, 2023 - pubs.rsc.org
Cytochrome P450s (Cyt P450s) and peroxidases are enzymes featuring iron heme cofactors
that have wide applicability as biocatalysts in chemical syntheses. Cyt P450s are a family of …

Strategies for stabilization of enzymes in organic solvents

V Stepankova, S Bidmanova, T Koudelakova… - Acs …, 2013 - ACS Publications
One of the major barriers to the use of enzymes in industrial biotechnology is their
insufficient stability under processing conditions. The use of organic solvent systems instead …

P450 BM3 (CYP102A1): connecting the dots

CJC Whitehouse, SG Bell, LL Wong - Chemical Society Reviews, 2012 - pubs.rsc.org
P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been
extensively studied over a period of almost forty years. The enzyme has been redesigned to …

Cytochromes P450 as versatile biocatalysts

R Bernhardt - Journal of biotechnology, 2006 - Elsevier
Cytochromes P450 are ubiquitously distributed enzymes, which were discovered about 50
years ago and which possess high complexity and display a broad field of activity. They are …

Enzymatic Hydroxylations of sp3-Carbons

J Munch, P Pullmann, W Zhang, MJ Weissenborn - Acs Catalysis, 2021 - ACS Publications
Enzymatic hydroxylation of activated and nonactivated sp3-carbons attracts keen interest
from the chemistry community as it is one of the most challenging tasks in organic synthesis …

Hemoproteins reconstituted with artificial metal complexes as biohybrid catalysts

K Oohora, A Onoda, T Hayashi - Accounts of Chemical Research, 2019 - ACS Publications
Conspectus In nature, heme cofactor-containing proteins participate not only in electron
transfer and O2 storage and transport but also in biosynthesis and degradation. The …

Cytochromes P450 as useful biocatalysts: addressing the limitations

E O'Reilly, V Köhler, SL Flitsch, NJ Turner - Chemical Communications, 2011 - pubs.rsc.org
Cytochrome P450 monooxygenases (P450s or CYPs) are a unique family of enzymes which
are capable of catalysing the regio-and stereospecific oxidation of non-functionalised …

Cytochrome P450 monooxygenases: perspectives for synthetic application

VB Urlacher, S Eiben - Trends in biotechnology, 2006 - cell.com
Cytochrome P450 monooxygenases are versatile biocatalysts that introduce oxygen into a
vast range of molecules. These enzymes catalyze diverse reactions in a regio-and …

Variations on a (t) heme—novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily

AW Munro, HM Girvan, KJ McLean - Natural product reports, 2007 - pubs.rsc.org
Covering: up to the end of 2006The catalytic potential of the P450s in organic
biotransformations is the subject of this review—with emphasis on the breadth of P450 redox …