Protein expression is a critical process in diverse biological systems. For Escherichia coli, a
widely employed microbial host in industrial catalysis and healthcare, researchers often face …

Escherichia coli has been widely used for the production of recombinant proteins. However,
the unbalances between host metabolism and recombinant biosynthesis continue to hamper …

Heterologous expression is the main approach for recombinant protein production ingenetic
synthesis, for which codon optimization is necessary. The existing optimization methods are …

Antibody drugs have been used to treat a number of diseases successfully. Producing
antibodies with high yield and quality is necessary for clinical applications of antibodies. For …

Herpesviruses infect a wide range of organisms including humans. Some of these viruses
are potential gene transfer vectors for gene therapy. However no study has been reported …

The evolution of bias in synonymous codon usage in chosen monkeypox viral genomes and
the factors influencing its diversification have not been reported so far. In this study, various …

Optimization of coding sequences to maximize protein expression yield is often outsourced
to external service providers during commercial gene synthesis and thus unfortunately …

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne viral zoonosis distributed
widely in Africa, Asia, Russia and the Balkans. The emergence and re-emergence of …

Peptide deformylase (PDF) is a metalloprotease catalyzing the removal of a formyl group
from newly synthesized proteins, which makes it an important antibacterial drug target …

The tRNA adaptation index (tAI) is a translation efficiency metric that considers weighted
values (S ij values) for codon–tRNA wobble interaction efficiencies. The initial …