Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics

SK Longo, MG Guo, AL Ji, PA Khavari - Nature Reviews Genetics, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) identifies cell subpopulations within tissue but
does not capture their spatial distribution nor reveal local networks of intercellular …

Brain organoids for the study of human neurobiology at the interface of in vitro and in vivo

I Chiaradia, MA Lancaster - Nature Neuroscience, 2020 - nature.com
Brain development is an extraordinarily complex process achieved through the spatially and
temporally regulated release of key patterning factors. In vitro neurodevelopmental models …

Conserved cell types with divergent features in human versus mouse cortex

RD Hodge, TE Bakken, JA Miller, KA Smith, ER Barkan… - Nature, 2019 - nature.com
Elucidating the cellular architecture of the human cerebral cortex is central to understanding
our cognitive abilities and susceptibility to disease. Here we used single-nucleus RNA …

Human hippocampal neurogenesis drops sharply in children to undetectable levels in adults

SF Sorrells, MF Paredes, A Cebrian-Silla, K Sandoval… - Nature, 2018 - nature.com
New neurons continue to be generated in the subgranular zone of the dentate gyrus of the
adult mammalian hippocampus,,,,. This process has been linked to learning and memory …

The human cell atlas

A Regev, SA Teichmann, ES Lander, I Amit, C Benoist… - elife, 2017 - elifesciences.org
The recent advent of methods for high-throughput single-cell molecular profiling has
catalyzed a growing sense in the scientific community that the time is ripe to complete the …

Organoid single-cell genomic atlas uncovers human-specific features of brain development

S Kanton, MJ Boyle, Z He, M Santel, A Weigert… - Nature, 2019 - nature.com
The human brain has undergone substantial change since humans diverged from
chimpanzees and the other great apes,. However, the genetic and developmental programs …

Comprehensive functional genomic resource and integrative model for the human brain

D Wang, S Liu, J Warrell, H Won, X Shi, FCP Navarro… - Science, 2018 - science.org
INTRODUCTION Strong genetic associations have been found for a number of psychiatric
disorders. However, understanding the underlying molecular mechanisms remains …

Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex

TJ Nowakowski, A Bhaduri, AA Pollen, B Alvarado… - Science, 2017 - science.org
Systematic analyses of spatiotemporal gene expression trajectories during organogenesis
have been challenging because diverse cell types at different stages of maturation and …

The next decade in AI: four steps towards robust artificial intelligence

G Marcus - arxiv preprint arxiv:2002.06177, 2020 - arxiv.org
Recent research in artificial intelligence and machine learning has largely emphasized
general-purpose learning and ever-larger training sets and more and more compute. In …

Single-cell spatial transcriptome reveals cell-type organization in the macaque cortex

A Chen, Y Sun, Y Lei, C Li, S Liao, J Meng, Y Bai, Z Liu… - Cell, 2023 - cell.com
Elucidating the cellular organization of the cerebral cortex is critical for understanding brain
structure and function. Using large-scale single-nucleus RNA sequencing and spatial …