Virus-induced gene silencing: empowering genetics in non-model organisms
AB Dommes, T Gross, DB Herbert… - Journal of …, 2019 - academic.oup.com
Virus-induced gene silencing (VIGS) is an RNA interference-based technology used to
transiently knock down target gene expression by utilizing modified plant viral genomes …
transiently knock down target gene expression by utilizing modified plant viral genomes …
Biolistic approach for transient gene expression studies in plants
Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells
via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an …
via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an …
[HTML][HTML] Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses
Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The
dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been …
dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been …
Agrobacterium-Mediated Gene Transient Overexpression and Tobacco Rattle Virus (TRV)-Based Gene Silencing in Cassava
H Zeng, Y ** countries. The completion of the cassava genome sequence and …
Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
D Tuo, Y Yao, P Yan, X Chen, F Qu, W Xue, J Liu… - Plant Methods, 2023 - Springer
Background Plant virus vectors designed for virus-mediated protein overexpression (VOX),
virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost …
virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost …
Stacking disease resistance and mineral biofortification in cassava varieties to enhance yields and consumer health
Delivering the benefits of agricultural biotechnology to smallholder farmers requires that
resources be directed towards staple food crops. To achieve effect at scale, beneficial traits …
resources be directed towards staple food crops. To achieve effect at scale, beneficial traits …
Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots
Key message The design and use of existing VIGS vectors for revealing monocot gene
functions are described and potential new vectors are discussed, which may expand their …
functions are described and potential new vectors are discussed, which may expand their …
Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration
Key message Among the five cassava isoforms (MeAPL1–MeAPL5), MeAPL3 is responsible
for determining storage root starch content. Degree of storage root postharvest physiological …
for determining storage root starch content. Degree of storage root postharvest physiological …
An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector
Z **ao, M **ng, X Liu, Z Fang, L Yang, Y Zhang… - Planta, 2020 - Springer
Main conclusion CaLCuV-based VIGS effectively works in cabbage and contributes to
efficient functional genomics research in Brassica crop species. Abstract Virus-induced gene …
efficient functional genomics research in Brassica crop species. Abstract Virus-induced gene …