The 26S proteasome is the most complex ATP-dependent protease machinery, of~ 2.5 MDa
mass, ubiquitously found in all eukaryotes. It selectively degrades ubiquitin-conjugated …

Endosomal protein complex required for transport-III (ESCRT-III) polymers are involved in
many crucial cellular functions, from cell division to endosome–lysosome dynamics. As a …

We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study
short-lived molecular states. Using piezoelectric dispensing, two independent streams of …

The cellular machine Cdc48 functions in multiple biological pathways by segregating its
protein substrates from a variety of stable environments such as organelles or multi-subunit …

ClpXP is an ATP-dependent protease in which the ClpX AAA+ motor binds, unfolds, and
translocates specific protein substrates into the degradation chamber of ClpP. We present …

Summary The hexameric Cdc48 ATPase (p97 or VCP in mammals) cooperates with its
cofactor Ufd1/Npl4 to extract polyubiquitinated proteins from membranes or macromolecular …

Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for
visualizing biological objects, which has been driven by advances across the entire pipeline …

Translocases of the AAA+ (ATPases Associated with various cellular Activities) family are
powerful molecular machines that use the mechano‐chemical coupling of ATP hydrolysis …

The p97 AAA+ ATPase is an essential and abundant regulator of protein homeostasis that
plays a central role in unfolding ubiquitylated substrates. Here we report two cryo-EM …

The endosomal sorting complexes required for transport (ESCRTs) are essential for multiple
membrane modeling and membrane-independent cellular processes. Here we describe six …