In all organisms, multi-subunit replicases are responsible for the accurate duplication of
genetic material during cellular division. Initiator proteins control the onset of DNA …

The replication origin and the initiator protein DnaA are the main targets for regulation of
chromosome replication in bacteria. The origin bears multiple DnaA binding sites, while …

Methods to enhance random mutagenesis in cells offer advantages over in vitro
mutagenesis, but current in vivo methods suffer from a lack of control, genomic instability …

The bacterial stringent response, triggered by nutritional deprivation, causes an
accumulation of the signaling nucleotides pppGpp and ppGpp. We characterize the …

Bacterial proliferation depends on the cells' capability to proceed through consecutive
rounds of the cell cycle. The cell cycle consists of a series of events during which cells grow …

The DNA of Escherichia coli contains 19,120 6-methyladenines and 12,045 5-
methylcytosines in addition to the four regular bases, and these are formed by the …

The replication period of Escherichia coli cells grown in rich medium lasts longer than one
generation. Initiation thus occurs in the 'mother‐'or 'grandmother generation'. Sister origins in …

The Escherichia coli SeqA protein contributes to regulation of chromosome replication by
preventing re-initiation at newly replicated origins. SeqA protein binds to new DNA which is …

Analogously to chromosome cohesion in eukaryotes, newly replicated DNA in E. coli is held
together by inter-sister linkages before partitioning into daughter nucleoids. In both cases …

ABSTRACT DNA segregation ensures that cell offspring receive at least one copy of each
DNA molecule, or replicon, after their replication. This important cellular process includes …