MicroRNAs in extracellular vesicles: Sorting mechanisms, diagnostic value, isolation, and detection technology

D Xu, K Di, B Fan, J Wu, X Gu, Y Sun… - … in bioengineering and …, 2022 - frontiersin.org
MicroRNAs (miRNAs) are a class of short, single-stranded, noncoding RNAs, with a length
of about 18–22 nucleotides. Extracellular vesicles (EVs) are derived from cells and play a …

Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens

Q Liu, X **, J Cheng, H Zhou… - Molecular Medicine …, 2023 - spandidos-publications.com
Infectious diseases are a major global cause of morbidity and mortality, seriously affecting
public health and socioeconomic stability. Since infectious diseases can be caused by a …

A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters

L Ma, J Wang, Y Li, D Liao, W Zhang, X Han… - Journal of hazardous …, 2023 - Elsevier
Highlights•We develop a versatile biosensing platform for ultrasensitive detection of
pathogenic bacteria, termed as SCENT-Cas.•SCENT-Cas adopts ratiometric fluorescence …

CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives

Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …

Mpox diagnostics: review of current and emerging technologies

CK Lim, J Roberts, M Moso, KC Liew… - Journal of Medical …, 2023 - Wiley Online Library
Mpox is a zoonotic disease caused by monkeypox virus (MPXV) from the Orthopoxvirus
genus. Unprecedented transmission events have led to more than 70 000 cases reported …

Advancements in visualizing loop-mediated isothermal amplification (LAMP) reactions: a comprehensive review of colorimetric and fluorometric detection strategies …

AS Sharma, NY Lee - Coordination Chemistry Reviews, 2024 - Elsevier
In the context of global disease conditions and the associated mortality attributed to
pathogens, nucleic acid amplification tests are crucial for precise and prompt diagnosis of …

[KNJIGA][B] Introduction to fluorescence sensing: Volume 2: Target recognition and imaging

AP Demchenko - 2023 - books.google.com
Fluorescence is the most popular technique in chemical and biological sensing because of
its ultimate sensitivity, high temporal and spatial resolution and versatility that enables …

A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin

BJ Yee, NF Shafiqah, NF Mohd-Naim… - International Journal of …, 2023 - Elsevier
This study introduces CRISPR/Cas-based aptasensor for the highly sensitive and specific
detection of the antibiotic, ampicillin. Ampicillin (AMPI) is a commonly used antibiotic for …

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

J Qiao, Z Zhao, Y Li, M Lu, S Man, S Ye… - Critical Reviews in …, 2024 - Taylor & Francis
Food safety problems have become one of the most important public health issues
worldwide. Therefore, the development of rapid, effective and robust detection is of great …

[HTML][HTML] Next-generation molecular diagnostics: Leveraging digital technologies to enhance multiplexing in real-time PCR

L Kreitmann, L Miglietta, K Xu… - TrAC Trends in …, 2023 - Elsevier
Real-time polymerase chain reaction (qPCR) enables accurate detection and quantification
of nucleic acids and has become a fundamental tool in biological sciences, bioengineering …