Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Recently, click chemistry has provided important advances in biomedical research fields.
Particularly, copper-free click chemistry including strain-promoted azide–alkyne …

In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

Chemistry, traditionally being the science of synthesis and structural manipulations of
molecules, has gradually undertaken the more challenging task of biology-oriented …

In all cells, proteins are continuously synthesized and degraded to maintain protein
homeostasis and modify gene expression levels in response to stimuli. Collectively, the …

The recent discovery of bioorthogonal click chemistry has created a new field in chemical
biology in which biomolecules that are not directly encoded in the genome can be monitored …

Highlights•∼ 2550 mRNA species are localized in dendrites and/or axons.•The alternative
usage of 3′ UTR isoforms dictates the fate of an mRNA molecule.•Novel multiplexed FISH …

Functional tools are needed to understand complex biological systems. Here we review how
chemical reporters in conjunction with bioorthogonal labeling methods can be used to …

Protein translation has been implicated in different forms of synaptic plasticity, but direct in
situ visualization of new proteins is limited to one or two proteins at a time. Here we describe …