Background Millions of children have been born throughout the world thanks to ARTs, the
harmlessness of which has not yet been fully demonstrated. For years, efforts to evaluate the …

In vitro follicle development (IVFD) is an adequate model to obtain basic knowledge of
folliculogenesis and provides a tool for ovarian toxicity screening. IVFD yielding competent …

Background Oocytes, the largest cells in mammals, harbor numerous mitochondria within
their cytoplasm. These highly dynamic organelles are crucial for providing energy resources …

High-quality, straightforward single-cell RNA sequencing (RNA-seq) with spatial resolution
remains challenging. Here, we developed DRaqL (direct RNA recovery and quenching for …

Ovarian organoids, based on mouse female germline stem cells (FGSCs), have great value
in basic research and are a vast prospect in pre-clinical drug screening due to their …

Postovulatory aging oocytes usually feature diminished potential for fertilization and poor
embryonic development due to enhanced oxidative damage to the subcellular organelles …

Human oogenesis is a highly complex and not yet fully understood process due to ethical
and technological barriers that limit studies in the field. In this context, replicating female …

STUDY QUESTION Is pronuclear transfer (PNT) capable of restoring embryo developmental
arrest caused by cytoplasmic inferiority of in vitro-grown (IVG) mouse oocytes? SUMMARY …

Today, fertility preservation is receiving more attention than ever. Cryopreservation, which
preserves ovarian tissue to preserve fertility in young women and reduce the risk of infertility …

The regulation of mRNA transcription and translation is uncoupled during oogenesis. The
reason for this uncoupling is two-fold. Chromatin is only accessible to the transcriptional …