CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Genome editing, which involves the precise manipulation of cellular DNA sequences to alter
cell fates and organism traits, has the potential to both improve our understanding of human …

Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary
cells remains a major challenge. Here we describe an engineered Peptide-Assisted …

Improving effector activity of antigen-specific T cells is a major goal in cancer
immunotherapy. Despite the identification of several effector T cell (T EFF)-driving …

Summary Interferon-gamma (IFN-γ) has pleiotropic effects on cancer immune checkpoint
blockade (ICB), including roles in ICB resistance. We analyzed gene expression in ICB …

CD8+ T cell exhaustion (Tex) limits disease control during chronic viral infections and
cancer. Here, we investigated the epigenetic factors mediating major chromatin-remodeling …

A lack of tools to precisely control gene expression has limited our ability to evaluate
relationships between expression levels and phenotypes. Here, we describe an approach to …

The chromatin reader eleven–nineteen leukemia (ENL) has been identified as a critical
dependency in acute myeloid leukemia (AML), but its therapeutic potential remains unclear …

The fetal-to-adult switch in hemoglobin production is a model of developmental gene control
with relevance to the treatment of hemoglobinopathies. The expression of transcription factor …

Genome editing is revolutionizing plant research and crop breeding. Sequence-specific
nucleases (SSNs) such as zinc finger nuclease (ZFN) and TAL effector nuclease (TALEN) …