Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

An outstanding challenge in single-molecule localization microscopy is the accurate and
precise localization of individual point emitters in three dimensions in densely labeled …

Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …

Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …

Metasurfaces have recently risen to prominence in optical research, providing unique
functionalities that can be used for imaging, beam forming, holography, polarimetry, and …

Fresnel incoherent correlation holography (FINCH) was a milestone in incoherent
holography. In this roadmap, two pathways, namely the development of FINCH and …