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Mass spectrometry using electrospray ionization
GRD Prabhu, ER Williams, M Wilm… - Nature Reviews Methods …, 2023 - nature.com
Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …
Mass spectrometry in proteomics
Proteomics can be viewed as an experimental approach to explain the information
contained in genomic sequences in terms of the structure, function, and control of biological …
contained in genomic sequences in terms of the structure, function, and control of biological …
[HTML][HTML] Electrospray ionization of large multiply charged species proceeds via Dole's charged residue mechanism
JF De La Mora - Analytica chimica acta, 2000 - Elsevier
The maximum charge zmax on a variety of electrospray ions of globular proteins retaining
their native structure, as well as naturally compact starburst dendrimers is compared to the …
their native structure, as well as naturally compact starburst dendrimers is compared to the …
Microdroplet fusion mass spectrometry for fast reaction kinetics
We investigated the fusion of high-speed liquid droplets as a way to record the kinetics of
liquid-phase chemical reactions on the order of microseconds. Two streams of micrometer …
liquid-phase chemical reactions on the order of microseconds. Two streams of micrometer …
Hydroxyl radical protein footprinting: a mass spectrometry-based structural method for studying the higher order structure of proteins
A McKenzie-Coe, NS Montes, LM Jones - Chemical reviews, 2021 - ACS Publications
Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …
successfully used to investigate a plethora of protein-related questions. The method, which …
Protein complexes in the gas phase: technology for structural genomics and proteomics
How does the development of mass spectrometry (MS) for the study of protein complexes,
intact in the gas phase, contribute to the twin fields of genomics and proteomics? Foremost …
intact in the gas phase, contribute to the twin fields of genomics and proteomics? Foremost …
Variable-temperature electrospray ionization for temperature-dependent folding/refolding reactions of proteins and ligand binding
Stabilities and structure (s) of proteins are directly coupled to their local environment or
Gibbs free energy landscape as defined by solvent, temperature, pressure, and …
Gibbs free energy landscape as defined by solvent, temperature, pressure, and …
Acid-Induced Unfolding of Cytochrome c at Different Methanol Concentrations: Electrospray Ionization Mass Spectrometry Specifically Monitors Changes in the …
L Konermann, DJ Douglas - Biochemistry, 1997 - ACS Publications
The acid-induced denaturation of ferricytochrome c (cyt c) was examined in aqueous
solutions containing different concentrations of methanol by electrospray ionization mass …
solutions containing different concentrations of methanol by electrospray ionization mass …
Studies of biomolecular conformations and conformational dynamics by mass spectrometry
Abstract I. Introduction 38 A. Protein Conformations and Dynamics 38 B. Protein‐Energy
Landscapes: The Folding Problem and Dynamics in the Native State 38 1. Protein …
Landscapes: The Folding Problem and Dynamics in the Native State 38 1. Protein …
Chaperonin function: folding by forced unfolding
M Shtilerman, GH Lorimer, S Walter Englander - Science, 1999 - science.org
The ability of the GroEL chaperonin to unfold a protein trapped in a misfolded condition was
detected and studied by hydrogen exchange. The GroEL-induced unfolding of its substrate …
detected and studied by hydrogen exchange. The GroEL-induced unfolding of its substrate …