Principles for integrative structural biology studies

MP Rout, A Sali - Cell, 2019 - cell.com
Integrative structure determination is a powerful approach to modeling the structures of
biological systems based on data produced by multiple experimental and theoretical …

Structural characterization of proteins and complexes using small-angle X-ray solution scattering

HDT Mertens, DI Svergun - Journal of structural biology, 2010 - Elsevier
Small-angle scattering of X-rays (SAXS) is an established method for the low-resolution
structural characterization of biological macromolecules in solution. The technique provides …

TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts

Y Shen, F Delaglio, G Cornilescu, A Bax - Journal of biomolecular NMR, 2009 - Springer
NMR chemical shifts in proteins depend strongly on local structure. The program TALOS
establishes an empirical relation between 13 C, 15 N and 1 H chemical shifts and backbone …

[PDF][PDF] 2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update

J Trewhella, AP Duff, D Durand, F Gabel… - … Section D: Structural …, 2017 - journals.iucr.org
In 2012, preliminary guidelines were published addressing sample quality, data acquisition
and reduction, presentation of scattering data and validation, and modelling for biomolecular …

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology

CA Brosey, JA Tainer - Current opinion in structural biology, 2019 - Elsevier
Highlights•SAXS captures architecture and dynamics to elucidate molecular
ensembles.•SAXS advances now probe key thermodynamic and kinetic properties of …

Small‐angle scattering for structural biology—Expanding the frontier while avoiding the pitfalls

DA Jacques, J Trewhella - Protein science, 2010 - Wiley Online Library
The last decade has seen a dramatic increase in the use of small‐angle scattering for the
study of biological macromolecules in solution. The drive for more complete structural …

[HTML][HTML] Methyl TROSY spectroscopy: a versatile NMR approach to study challenging biological systems

S Schütz, R Sprangers - Progress in nuclear magnetic resonance …, 2020 - Elsevier
A major goal in structural biology is to unravel how molecular machines function in detail. To
that end, solution-state NMR spectroscopy is ideally suited as it is able to study biological …

SAXS ensemble refinement of ESCRT-III CHMP3 conformational transitions

B Różycki, YC Kim, G Hummer - Structure, 2011 - cell.com
We developed and implemented an ensemble-refinement method to study dynamic
biomolecular assemblies with intrinsically disordered segments. Data from small angle X-ray …

Critical assessment of methods of protein structure prediction: Progress and new directions in round XI

J Moult, K Fidelis, A Kryshtafovych… - Proteins: Structure …, 2016 - Wiley Online Library
Modeling of protein structure from amino acid sequence now plays a major role in structural
biology. Here we report new developments and progress from the CASP11 community …

Target flexibility: an emerging consideration in drug discovery and design

P Cozzini, GE Kellogg, F Spyrakis… - Journal of medicinal …, 2008 - ACS Publications
Structure-based drug discovery has played an important role in medicinal chemistry, 1
beginning nearly when the first X-ray crystal structure of the myoglobin and hemoglobin …