Single-molecule experiments in biological physics: methods and applications
F Ritort - Journal of Physics: Condensed Matter, 2006 - iopscience.iop.org
I review single-molecule experiments (SMEs) in biological physics. Recent technological
developments have provided the tools to design and build scientific instruments of high …
developments have provided the tools to design and build scientific instruments of high …
Biomolecules under mechanical force
Recent advances in single molecule experiments have raised many challenges. These
challenges can be met by a proper understanding of the inter-and intra-molecular …
challenges can be met by a proper understanding of the inter-and intra-molecular …
Molecular mechanocytometry using tension-activated cell tagging
Flow cytometry is used routinely to measure single-cell gene expression by staining cells
with fluorescent antibodies and nucleic acids. Here, we present tension-activated cell …
with fluorescent antibodies and nucleic acids. Here, we present tension-activated cell …
Ultrafast hole relaxation dynamics in quantum dots revealed by two-dimensional electronic spectroscopy
Elucidating the population dynamics of correlated electron-hole pairs (bound excitons) in
semiconducting quantum dots (QDs) is key for develo** our fundamental understanding of …
semiconducting quantum dots (QDs) is key for develo** our fundamental understanding of …
Unraveling the structure of DNA during overstretching by using multicolor, single-molecule fluorescence imaging
Single-molecule manipulation studies have revealed that double-stranded DNA undergoes
a structural transition when subjected to tension. At forces that depend on the attachment …
a structural transition when subjected to tension. At forces that depend on the attachment …
Tension gauge tethers as tension threshold and duration sensors
Mechanotransduction, the process by which cells respond to tension transmitted through
various supramolecular linkages, is important for understanding cellular behavior. Tension …
various supramolecular linkages, is important for understanding cellular behavior. Tension …
Revealing the competition between peeled-Ssdna, melting bubbles and S-DNA during DNA overstretching using fluorescence microscopy
By using a dual laser optical tweezers with a fast force feedback, we were able to record the
exponential elongation following force steps imposed on D-phage ds-DNA molecule and on …
exponential elongation following force steps imposed on D-phage ds-DNA molecule and on …
Biophysical characterization of DNA binding from single molecule force measurements
Single molecule force spectroscopy is a powerful method that uses the mechanical
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …
Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching by single-molecule calorimetry
Double-stranded DNA (dsDNA) unconstrained by torsion undergoes an overstretching
transition at about 65 pN, elongating the DNA to about 1.7-fold. Three possible structural …
transition at about 65 pN, elongating the DNA to about 1.7-fold. Three possible structural …
Torque and dynamics of linking number relaxation in stretched supercoiled DNA
JF Marko - Physical Review E—Statistical, Nonlinear, and Soft …, 2007 - APS
In micromechanical studies of DNA, plectonemically supercoiled domains are often used as
sources of constant torque. These torques are not easily measured and are instead usually …
sources of constant torque. These torques are not easily measured and are instead usually …